【摘要】 杂交育种是杧果新品种选育最重要最有效的手段，快速高效鉴定杂交后代真实性是实现育种计划的前提。以“金煌（Mangifera indica L．‘Jinhuang’）”ד热农1号（M.indica L．‘Renong No.1’）”、“金煌”ד爱文（M.indica L．‘Irwin’）”、“热农1号”ד台农1号（M.indica L．‘Tainong No.1’）”杂交后代及亲本为试材，采用荧光SSR标记结合毛细管电泳技术筛选亲本间具有多态性的SSR引物，对其杂交后代单株真实性进行鉴定。结果表明，“金煌”ד热农1号”杂交F₁的7个单株在7个位点上，“金煌”ד爱文”杂交F₁的6个单株在6个位点上，“热农1号”ד台农1号”杂交F₁的11个单株在4个位点上扩增出双亲的多态性条带，鉴定为真杂种。UPGMA聚类分析中，141132、141193与“金煌”聚成一类，1411100、130405和141166与“热农1号”聚成一类，而141182和141195分别单独为一类；“金煌”ד爱文”和“热农1号”ד台农1号”的F₁单株均与父本聚为一类，表现出偏父本遗传的倾向。研究结果为利用荧光SSR分子标记进行杧果杂交种真实性鉴定提供重要参考。更多还原

【Abstract】 Hybrid breeding is the most important and effective method for developing new mango varieties,and the rapid and efficient identification of hybrid progeny is a prerequisite for the implementation of breeding programs.In this study,the hybrid progeny and their parents from three crosses including ’Jinhuang’× ’Renong No.1’,’Jinhuang’× ’Irwin’,and ’Renong No.1’× ’Tainong No.1’ were used as experimental materials.With capillary electrophoresis,fluorescent polymorphic SSR markers were screened between the parents and further used to identify their hybrid progeny.The results showed that seven individuals of the’Jinhuang’× ’Renong No.1’ cross at seven loci,six individuals of the’Jinhuang’× ’Irwin’cross at six loci,and eleven individuals of the ’Renong No.1’× ’Tainong No.1’ cross at four loci amplified polymorphic bands from both parents,indicating all of them were hybrids.In the UPGMA clustering analysis,141132 and 141193 were clustered with’Jinhuang’,1411100,130405,and 141166were clustered with’Renong No.1’,while 141182 and 141195 were clustered in a separate category.All F₁ individuals from both ’Jinhuang’× ’Irwin’and ’Renong No.1’× ’Tainong No.1’ crosses were clustered with their respective paternal parents,showing a tendency of paternal inheritance.The results of this study provide important theoretical basis and reference for the identification of mango hybrids using fluorescent SSR molecular markers.更多还原