【摘要】 组蛋白去乙酰化酶ZmHDT102(Zea mays L., Histone Deacetylase, HDT102为玉米HDAC家族系统编号)与拟南芥AtHD2C、水稻OsHDT701为直系同源基因，均具有高度保守的HDAC结构域。鉴于该类基因在植物发育调控中的关键作用，本研究聚焦于ZmHDT102在玉米中的功能解析，通过生物信息分析发现，ZmHDT102在玉米生殖发育相关组织，如成熟种子、胚乳和胚中，呈现出时空特异性高表达模式。为进一步探究其功能，本研究利用3种功能缺失型突变体：hdt102-1（起始密码子ATG突变）、hdt102-2（编码区错义突变）和hdt102-3(Mu转座子插入突变)，结合DNA测序、qRT-PCR(Quantitative reverse transcription PCR)和Western blot验证，证实突变体中ZmHDT102的转录水平（降至野生型的30%～50%）与蛋白水平均显著降低。表型分析显示，突变体在营养生长阶段呈现株高降低、茎粗变细的表型缺陷；生殖生长阶段则表现为果穗长度缩短、籽粒体积减小及千粒重显著下降的形状改变。这些结果表明，ZmHDT102通过正调控玉米植株形态建成、穗部发育及籽粒充实过程，在玉米生长发育中发挥关键作用。本研究不仅明确了ZmHDT102在玉米中的具体功能，更为揭示组蛋白乙酰化修饰调控玉米生长发育的分子机制提供了关键实验证据。更多还原

【Abstract】 Histone deacetylase ZmHDT102(Zea mays L., Histone Deacetylase, HDT102 is the maize HDAC family system number), Arabidopsis AtHD2C and rice OsHDT701 are orthologous with highly conserved HDAC domains. In view of the key role of these genes in plant development regulation, this study focused on the functional analysis of ZmHDT102 in maize. Through bioinformatics analysis, it was found that ZmHDT102 showed a with high expression pattern spatio-temporally specificity in maize reproductive development-related tissues, such as mature seeds, endosperm and embryo. In order to further explore its function, three functional deletion mutants: hdt102-1(initiation codon ATG mutation), hdt102-2(coding region missense mutation), and hdt102-3(Mu transposon insertion mutation) were used in this study. Combined with DNA sequencing, qRT-PCR(quantitative reverse transcription PCR) and Western blot verification, it was confirmed that the transcription level(reduced to 30 %-50 % of the wild type) and protein level of ZmHDT102 in the mutant were significantly reduced. Phenotypic analysis showed that the mutant showed phenotypic defects of plant height reduction and stem diameter thinning at the vegetative growth stage. In the reproductive growth stage, the ear length was shortened, the grain volume was reduced, and the 1 000-grain weight was significantly reduced. These results indicate that ZmHDT102 plays a key role in maize growth and development by positively regulating maize plant morphogenesis, panicle development and grain filling. This study not only clarified the specific function of ZmHDT102 in maize, but also provided key experimental evidence for revealing the molecular mechanism of histone acetylation modification regulating maize growth and development.更多还原