【摘要】 目的 探讨种子育麟散对控制性超促排卵大鼠的子宫内膜白血病抑制因子（LIF）、基质金属蛋白酶-9（MMP-9）和基质金属蛋白酶抑制因子-1（TIMP-1）表达水平的影响，分析种子育麟散治疗不孕症的可能研究机制。方法 选用40只雌性SD大鼠，随机分成空白对照组、模型组、阿司匹林对照组、中药组，每组10只。制备控制性超促排卵大鼠模型：腹腔内注射丙氨瑞林、尿促性腺激素及注射用绒毛膜促性腺激素。中药组给予种子育麟散7.2 g·kg^-1·d^-1灌胃，阿司匹林组予0.2 g·kg^-1·d^-1阿司匹林溶液灌胃，同时予空白对照组、模型组0.9%氯化钠溶液灌胃。于孕5 d,采用免疫组化法、Western blot分别检测大鼠子宫LIF、MMP-9、TIMP-1蛋白表达水平，RT-qPCR法检测LIF、MMP-9、TIMP-1 mRNA表达水平。结果 与空白对照组比较，模型组子宫内膜LIF、MMP-9、TIMP-1蛋白和mRNA的表达水平明显降低（P<0.05）;与模型组比较，阿司匹林组、中药组子宫内膜LIF、MMP-9、TIMP-1蛋白和mRNA的表达水平明显升高（P<0.05）。结论 种子育麟散可能通过调节子宫内膜LIF、MMP-9、TIMP-1表达水平来改善子宫内膜容受性，可为不孕症的治疗提供依据。更多还原

【Abstract】 Objective To investigate the impact of Zhongziyulin Powder（ZZYP） on endometrial leukemia inhibitory factor（LIF）,matrix metalloproteinase-9（MMP-9） and tissue inhibitor of metalloproteinase-1（TIMP-1） in endometrium of controlled super promoting ovulation rats.Methods Forty female Sprague-Dawley（SD） rats were selected and randomly divided into the blank control group, model group, aspirin control group and Chinese medicine group, 10 cases in each group.The controlled ovarian hyperstimulation（COH） rat model was established: intraperitoneal injections of alarelin, urinary gonadotropin and chorionic gonadotropin for injection.The Chinese medicine group was given ZZYP 7.2 g·kg^-1·d^-1 by gavage.The aspirin group was given 0.2 g·kg^-1·d^-1 aspirin solution by gavage, meanwhile the blank control group and model group were given 0.9% sodium chloride solution by gavage.On 5 d of gestation, the immunohistochemistric method and Western blot were used to respectively detect the expression of LIF,MMP-9 and TIMP-1 proteins expressions in the rat endometrium, while the mRNA expressions of LIF,MMP-9 and TIMP-1 were measured by RT-qPCR.Results Compared with the blank control group, the LIF,MMP-9 and TIMP-1 protein and mRNA expression levels in the model group were significantly decreased（P<0.05）.Compared with the model group, the LIF,MMP-9 and TIMP-1 protein and mRNA expression levels in the endometrium of the aspirin group and ZZYP group were significantly increased（P<0.05）.Conclusion ZZYP may improve the endometrial receptivity by regulating the expressions of LIF,MMP-9 and TIMP-1 protein and mRNA,which could provide the basis for treating infertility.更多还原