【摘要】 壳聚糖基薄膜在食品保鲜中具有绿色可降解的优势，但其脆性和功能单一问题限制了应用。为此，提出以甘油-氯化胆碱组成的低共熔溶剂（DES）为增塑剂、CaCl₂为交联剂，构建了多功能壳聚糖基复合膜及涂膜体系。当甘油-氯化胆碱摩尔比为1.5:1.0、Ca²⁺质量分数为2%时，复合膜的断裂伸长率达142.96%且遮光能力显著增强。通过浸渍的方式进行保鲜性能测试，结果表明涂膜处理可有效抑制草莓失重率，贮藏至第5天时其硬度与可溶性固形物保持更优水平，且霉斑产生时间延缓2 d。Ca²⁺可通过配位壳聚糖氨基形成交联网络，协同DES的氢键作用增强薄膜韧性，同时激活果实钙调素（CaM）信号通路，维持细胞壁完整性。所设计的壳聚糖膜为绿色高效保鲜材料。更多还原

【Abstract】 Chitosan-based films exhibit green and biodegradable advantages in food preservation, but the brittleness and single function limit their practical applications. A multifunctional chitosan composite film and coating system was constructed using a deep eutectic solvent（DES） composed of glycerol and choline chloride as a plasticizer and CaCl₂ as a crosslinker. It was found that when the molar ratio of glycerol to choline chloride was 1.5:1.0 and the Ca²⁺ concentration was 2%, the fracture elongation of the composite film reached 142.96%, and its light-shielding ability was significantly enhanced. Through immersion coating for preservation performance testing, the results showed that the coating treatment could effectively inhibit the weight loss of strawberries. By the 5 th day of storage, the hardness and soluble solids maintained at a better level, and the time of mold spot formation was delayed by 2 days. Ca²⁺ formed a crosslinked network by coordinating with chitosan amino groups, synergistically enhancing film toughness with the hydrogen bonding effect of DES. Meanwhile, it activated the calcium/calmodulin-dependent protein kinase（CaM） signaling pathway in fruits, maintaining cell wall integrity. The rational design of chitosan films provides theoretical support and practical references for the development of green and efficient preservation materials.更多还原