【摘要】 目的:探讨杞菊地黄汤(QJDHT)对去势所致的干眼大鼠炎症损伤和辅助性T细胞17/调节性T细胞(Th17/Treg)免疫平衡的影响。方法:将50只健康大鼠随机分为对照组、模型组、QJDHT低剂量(QJDHT-L)组、QJDHT高剂量(QJDHT-H)组以及睾酮组,除对照组外均行双侧睾丸及附睾切除术,术后饲养6个月,期间监测大鼠泪液分泌量和病理形态变化。待干眼模型形成后,各组给药28 d。随后采用泪液分泌实验和角膜荧光素(FL)染色观察眼表体征;HE染色法观察大鼠泪腺病理形态变化;流式分析仪观察外周血Th17/Treg细胞比例;Western Blot检测白细胞介素17 (IL-17)、白细胞介素6(IL-6)、转化生长因子β 1(TGF-β 1)、叉头框蛋白P3(FOXP3)蛋白表达水平。结果:与模型组比较,经QJDHT药物干预后,干眼大鼠的泪液分泌量显著增多(P<0.01),FL评分显著降低(P<0.01),泪腺腺泡排列更为有序,炎性细胞和淋巴细胞浸润相对减少,外周血Th17/Treg细胞比例显著降低(P<0.01),同时IL-17和IL-6蛋白表达显著降低(P<0.01),TGF-β及FOXP3蛋白表达显著升高(P<0.05,P<0.01)。结论:QJDHT可通过纠正Th17/Treg免疫失衡,减轻去势所致干眼大鼠的炎症损伤,发挥抗炎抗免疫作用。更多还原

【Abstract】 Objective:To investigate the effects of Qiju Dihuang Decoction(QJDHT) on inflammatory and Th17/Treg immune balance in castration-induced dry eye rats.Methods:A total of 50 healthy rats were randomly divided into control,model,low-dose QJDHT(QJDHT-L),high-dose QJDHT(QJDHT-H) and testosterone groups.Bilateral testicular and epididymal resection was performed in all rats except the control group.After 6 months,tear secretion and pathological morphological changes were monitored.After the formation of dry eye model,treatments were administered for 28 days.Schirmer’ s test and corneal fluorescein(FL) staining were used to observe the ocular surface signs,HE staining was used to observe the pathological morphological changes of the lacrimal glands.Flow cytometry measured Th17/Treg cell ratios in peripheral blood,and Western Blot quantified the expression of interleukin-6(IL-6),interleukin-17(IL-17),transforming growth factor-β1(TGF-β1),and forkhead box protein P3(FOXP3).Results:Compared with the model group,QJDHT intervention significantly increased tear production(P<0.01),and FL scores in dry eye rats significantly decreased(P<0.01),lacrimal acinus were more orderly arranged,inflammatory cells and lymphocyte infiltration decreased,and the proportion of Th17/Treg cells in peripheral blood significantly decreased in dry eye rats(P<0.01),at the same time,the protein expression of IL-17 and IL-6 significantly decreased(P<0.01),while the protein expression of TGF-β and FOXP3 significantly increased(P<0.05,P<0.01).Conclusion:QJDHT can correct Th17/Treg immune imbalance,reduce the inflammatory damage in castration-induced dry eye,and play an anti-inflammatory and anti-immune role.更多还原