【摘要】 为明确白藜芦醇（Resveratrol,Res）对核桃主要过敏原Jug r 1致敏性的影响及作用机制，采用凝胶过滤层析技术纯化核桃过敏原Jug r 1，并构建C57BL/6小鼠过敏模型，结合分子对接、傅里叶变换红外光谱（Fourier-transform infrared spectroscopy,FT-IR）等方法，探讨Res对Jug r 1结构及致敏性的影响。结果表明，核桃蛋白加入Res后，可显著缓解小鼠过敏症状，降低血清免疫球蛋白E(Immunoglobulin E,IgE)、组胺（Histamine,HIS）及小鼠肥大细胞蛋白酶-1(Mouse mast cell protease-1,mMCP-1)水平，上调Th1型细胞因子小鼠γ干扰素（Interferon-γ,IFN-γ）的表达，下调Th2型细胞因子白细胞介素-4(Interleukin-4,IL-4)、白细胞介素-5(Interleukin-5,IL-5)的分泌，并对核桃过敏小鼠空肠及脾脏组织具有保护作用；白藜芦醇与Jug r 1的最低结合能为-5.637 kcal/mol，可与Q59、Y55、Q70、R71位点形成氢键，与F74位点产生π-π共轭作用；通过FT-IR证实Res通过氢键与疏水作用改变蛋白高级结构。Res可通过改变Jug r 1的高级结构，纠正Th1/Th2免疫失衡，从而降低其致敏性，为低敏核桃制品的开发提供理论基础与试验依据。更多还原

【Abstract】 To clarify the regulatory mechanism of resveratrol(Res) on the structure and allergenicity of the major walnut allergen Jug r 1, Jug r 1 was purified by gel filtration chromatography, and a C57 BL/6 mouse allergy model was established. Structural and allergenic changes were analyzed using molecular docking and Fourier-transform infrared spectroscopy(FT-IR). Results demonstrated that Res significantly relieved allergic symptoms, reduced serum levels of IgE, histamine(HIS), and mMCP-1, up-regulated IFN-γ expression, and down-regulated IL-4 and IL-5 secretion. Res also protected the jejunum and spleen tissues of allergic mice. The lowest binding energy between resveratrol and Jug r 1 is-5.637 kcal/mol, with hydrogen bonds formed at residues Q59, Y55, Q70, and R71, and π-π stacking with residue F74. FT-IR confirmed that Res altered the higher-order structure of the protein through hydrogen bonding and hydrophobic interactions. Res lowers Jug r 1 allergenicity by modifying its higher-order structure and rebalancing Th1/Th2 immunity, which provides a theoretical and experimental basis for developing hypoallergenic walnut products.更多还原