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硫氢化钠对采后无花果一氧化氮和硫化氢代谢的影响

Effect of Sodium Hydrosulfide on the Metabolism of Nitric Oxide and Hydrogen Sulfide in Postharvest Figs

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【作者】 刘子萱; 毛心悦; 朱树华; 李成坤;

【Author】 LIU Zixuan;MAO Xinyue;ZHU Shuhua;LI Chengkun;College of Chemistry and Materials Science, Shandong Agricultural University;

【通讯作者】 李成坤;

【机构】 山东农业大学化学与材料科学学院;

【摘要】 为探究硫氢化钠(NaHS)处理对采后无花果的保鲜效果,以蒸馏水熏蒸处理为对照,采用NaHS溶液和亚牛磺酸(HT)溶液进行熏蒸处理,研究果实采后可溶性固形物含量、相对电导率、色差、失重率、硬度、内源一氧化氮(NO)含量、内源硫化氢(H2S)含量、L-精氨酸(L-Arg)含量、一氧化氮合酶(NOS)活性、亚硝酸盐(含量、硝酸还原酶(NR)活性、L-半胱氨酸脱巯基酶(L-CD)活性、丝氨酸乙酰转移酶(SAT)活性及O-乙酰丝氨酸硫裂解酶(OAS-TL)活性指标的变化。结果表明:与对照组相比,NaHS处理可提高果实可溶性固形物含量,抑制相对电导率、色差和失重率的上升,减缓果实硬度下降,提高NR活性,促进N转化为NO,有利于内源NO的累积,而HT处理则呈现相反的结果。此外,两种处理均可提高果实内源H2S含量,但NaHS处理效果更好,其通过提高L-CD活性促进内源H2S含量增加,HT处理则通过提高SAT和OAS-TL活性提升内源H2S含量。总体而言,NaHS处理效果最佳,有助于提升无花果保鲜效果,改善无花果的贮藏品质。本研究结果可为采后无花果的保鲜提供技术与理论支撑。

【Abstract】 To investigate the preservation impact of sodium hydrosulfide(NaHS) treatment on the postharvest figs, fumigation with NaHS solution and hypotaurine(HT) solution were conducted, using distilled water fumigation as a control. The changes in soluble solid content, relative conductivity, chromatism, weight loss rate, firmness, endogenous nitric oxide(NO) content, endogenous H2S content, L-Arginine(L-Arg) content, nitric oxide synthase(NOS) activity, nitrite( content, nitrate reductase(NR) activity, L-cysteine desulfhydrase(L-CD) activity, serine acetyltransferase(SAT) activity, and O-acetylserine sulfhydrylase(OAS-TL) activity in the fruit were tested. The results indicated that, compared with the control treatment, NaHS treatment raised the soluble solid content, restrained the relative conductivity, chromatism, and weight loss rate, delayed the decline of fruit firmness, enhanced NR activity, promoted N to NO conversion, and facilitated the endogenous NO accumulation, while HT treatment showed opposite effects. In addition, both treatments increased the endogenous H2S content in the fruits, but NaHS treatment was more effective, as it enhanced the accumulation of endogenous H2S by increasing L-CD activity, while HT treatment improved the endogenous H2S content by raising SAT and OAS-TL activities. Overall, NaHS treatment demonstrates superior efficacy in improving storage quality and exhibits a more pronounced preservation effect. The findings of this study provide technical and theoretical support for the preservation of postharvest figs.

【关键词】 无花果; 采后; 贮藏; NaHS处理; NO代谢; H2S代谢;
【Key words】 fig; postharvest; storage; NaHS treatment; NO metabolism; H2S metabolism;
【基金】 山东省重点研发计划项目(2022TZXD0023)
  • 【文献出处】 保鲜与加工 ,Storage and Process , 编辑部邮箱 ,2026年01期
  • 【分类号】TS255.3
  • 【下载频次】17
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