【摘要】 反式阿魏酸（TFA）是一种具有抗炎、抗氧化及抗肿瘤活性的天然酚酸类化合物，其在复杂生物发酵液中的高效分离面临挑战。基于磺酸化氧化石墨烯（SGO）和分子印迹聚合物（MIP）制备了SGO/MIP复合膜，通过双功能单体（甲基丙烯酸和丙烯酰胺）构建特异性识别位点，结合非溶剂致相分离法（NIPS）调控膜孔结构，可实现TFA的高效分离。结果显示：在SGO、MIP、聚偏二氟乙烯（PVDF）、聚乙二醇2000（致孔剂）的质量比为0.9∶0.225∶12∶0.72条件下制备的SGO/MIP复合膜，在25℃下对发酵液中TFA的吸附容量达2 249.31 mg/m~2，膜通量为53.94L/（m~2·h），表明其在高效截留目标分子的同时保持了良好的渗透性能；使用10%～60%（体积分数）乙醇进行梯度洗脱并重结晶后，TFA纯度为96.4%，回收率为87.17%。本研究通过引入SGO和双功能单体策略，显著提高了目标分子的选择性吸附能力，为天然产物的规模化分离提供了新思路。更多还原

【Abstract】 Trans-ferulic acid(TFA) is a natural phenolic acid compound with anti-inflammatory, antioxidant andantitumor activities. Its efficient separation in complex biological fermentation broths is challenging. In this study, sulfonated graphene oxide(SGO)/molecularly imprinted polymers(MIP) composite membranes were prepared. Specific recognition sites were constructed using bifunctional monomers(methacrylic acid and acrylamide), and the membrane pore structure was regulated by non-solvent induced phase separation(NIPS), which enabled efficient separation of TFA. The results show that the SGO/MIP composite membrane prepared with mass ratio of SGO∶MIP∶polyvinylidene fluoride(PVDF)∶polyethylene glycol 2000(pore-forming agent) of 0. 9∶0. 225∶12∶0. 72 has an adsorption capacity of 2 249. 31 mg/m~2 for TFA in the fermentation broth at 25 ℃, and a membrane flux of 53. 94 L/（m~2·h）, indicating that it maintains good permeation performance while efficiently retaining target molecules. After gradient elution with 10%-60%(volume fraction) ethanol and recrystallization, the purity of TFA was 96. 4% and the recovery rate was 87. 17%. This study shows that the selective adsorption capacity of target molecules is significantly improved by introducing SGO and bifunctional monomer strategies, which provides a new idea for the large-scale separation of natural products.更多还原