【摘要】 [目的]为紫薇（Lagerstroemia indica）生物育种和基因功能研究提供技术。[方法]以紫薇品种“千层绯雪”叶片和“缤粉佳人”茎段为外植体，以GFP、RUBY为报告基因，研究了外植体类型、抗生素浓度、菌液浓度、侵染时间、共培养时间等因素对遗传转化的影响。[结果]以叶片为外植体的遗传转化未获得阳性植株，侵染“缤粉佳人”茎段后，在预培养1 d、OD₆₀₀值为1.2～1.5、侵染时间为5～15 min、共培养2 d的条件下得到转基因阳性植株，经PCR检测证明成功获得转基因植株。[结论]紫薇茎段转基因体系的建立为紫薇基因功能分析和基因编辑研究提供了技术支撑。更多还原

【Abstract】 [Objective]To provide a technical basis for molecular breeding and gene function evaluation of Lagerstroemia indica.[Method]The effects of antibiotic concentration, explant type, bacterial concentration, infection time and co-culture time on genetic transformation were investigated using leaves of L.indica “Qiancengfeixue” and stem segments of L.indica “Binfenjiaren” as explants, GFP and RUBY were used as reporter genes.[Result]Transgenic plants were not successfully obtained by leaf transformation.After infecting stem segments of “Binfenjiaren”,positive transgenic plants were obtained when pre-culture was 1 day, OD₆₀₀ was 1.2-1.5,infection time was 5-15 min, and co-culture time was 2 days.[Conclusion]The established genetic transformation system using L.indica stem segments provides technical support for functional analysis and gene editing of L.indica genes.更多还原