【摘要】 [目的]明确珠芽蓼中驱动小金蝠蛾幼虫取食的核心信号物质。[方法]通过对珠芽蓼进行取食分组(偏好取食组QS/非取食组FQS),结合代谢组学、取食偏好试验及幼虫增重试验,解析珠芽蓼中影响幼虫取食定位的关键信号物质。[结果]在有取食差异的珠芽蓼中共鉴定出2 942种差异代谢物,其中次生代谢途径是差异代谢物优先富集的途径,其次是氨基酸与核苷酸代谢;核心差异代谢物包括α-岩藻糖、氨基葡萄糖-6-磷酸、L-苹果酸、1,6-脱水-β-葡萄糖等,且L-苹果酸在FQS组显著降低(P<0.001);取食偏好试验显示,在珠芽蓼中添加L-苹果酸后取食选择率超48%、人参果(Argentina anserina)添加组超55%,均显著高于未添加组(P<0.05),甚至在椰糠中单独添加L-苹果酸亦可显著吸引幼虫驻留栖息(P<0.05);幼虫增重试验表明,添加L-苹果酸的饲料饲养90 d后,幼虫增重(0.20 g)显著高于未添加组(0.14 g,P<0.05)。[结论]该研究为蝙蝠蛾幼虫人工饲料体系的建立提供科学依据。更多还原

【Abstract】 [ Objective] To identify key signaling substances driving feeding behavior of Hepialus xiaojinensis larvae in Bistorta vivipara.[Method] B. vivipara was divided into feeding groups(preferred feeding group QS/non-feeding group FQS). Combined with metabolomics analysis, feeding preference tests, and larval weight gain tests, the key signaling substances in B. vivipara that affect larval feeding localization were identified. [Result] The results showed that: a total of 2 942 differential metabolites were identified between B. vivipara groups with different feeding preferences; among these, differential metabolites were preferentially enriched in secondary metabolic pathways, followed by amino acid and nucleotide metabolism. Core differential metabolites included α-fucose, glucosamine 6-phosphate, L-malic acid, and 1,6-anhydro-β-glucose. The content of L-malic acid in the FQS group decreased sharply(P<0. 001). Feeding preference tests showed that after adding L-malic acid to B. vivipara, the larval feeding selection rate exceeded 48%, and it exceeded 55% when added to Argentina anserina(alternative diet); both rates were significantly higher than those of the non-added groups(P<0. 05). Even when L-malic acid was added alone to cocopeat(non-food substrate), it significantly induced larval residence and feeding(P<0. 05). Larval weight gain tests indicated that after 90 d of rearing with diets supplemented with L-malic acid, the larval weight gain(0. 20 g) was significantly higher than that of the nonadded group(0. 14 g, P<0. 05). [Conclusion] The study provides a scientific basis for the establishment of an artificial diet system for Hepiallus larvae.更多还原