【摘要】 [目的]研究烟草黑胫病发生对根际土壤细菌和真菌群落的影响，探明影响根际土壤微生物群落的环境因子。[方法]通过宏基因组测序比较健康和感病烟株根际土壤细菌和真菌群落，解析微生物群落与环境因子的关系。[结果]与健康土壤群落相比，感病土壤真菌群落多样性增加，而真菌和细菌群落丰富度降低。群落组成差异分析表明，健康烟株土壤中细菌群落鞘脂单孢菌属(Sphingomonas)等益生菌丰度增加，而纤线杆菌属(Ktedonobacter)丰度降低，健康烟株土壤中真菌群落根鞘霉属(Rhizophagus)丰度增加，而曲霉属(Aspergillus)、根霉属(Rhizopus)丰度降低。碱解氮、有效磷、有机质与健康烟株根际土壤细菌群落呈正相关，真菌门群落水平相关性分析发现，速效钾、碱解氮与健康烟株根际土壤真菌群落呈正相关。[结论]与健康根际土壤相比，感病根际土壤细菌和真菌群落丰富度、有益菌丰度、有机质、碱解氮、有效磷含量降低，而真菌群落多样性增加。更多还原

【Abstract】 [Objective]To study the effect of tobacco black shank disease occurrence on rhizosphere soil bacterial and fungal communities, and to identify environmental factors affecting inter-root soil microbial communities. [Method]Comparison of rhizosphere soil bacterial and fungal communities in healthy and susceptible tobacco plants by meta-genomic sequencing to analyze the relationship between microbial communities and environmental factors. [Result] Fungal community diversity increased compared with healthy soil communities, while fungal and bacterial communities decreaseed. Analysis of differences in community composition showed that the abundance of probiotic bacteria such as Sphingomonas increased while Ktedonobacter decreased, and the abundance of fungal community Rhizophagus increased while Aspergillus and Rhizopus decreased in the soil of healthy tobacco plants.There was a positive correlation between alkaline dissolved nitrogen, effective phosphorus, and organic matter and rhizosphere soil bacterial communities of healthy tobacco plants, and the results of correlation analysis at the level of fungal phylum communities revealed that there was a positive correlation between fast-acting potassium, alkaline dissolved nitrogen, and rhizosphere soil fungal communities of healthy tobacco plants. [Conclusion] Compared with healthy rhizosphere soil, the abundance of bacteria and fungal communities, beneficial bacterial abundance, organic matter, alkaline nitrogen and effective phosphorus content decreased, while the diversity of fungal community increased.更多还原