【摘要】 苹果RR基因家族在苹果的生长过程中扮演着重要角色，探究MdRR基因在苹果中的进化及其生物学特征具有重要意义。运用生物信息学的方法，在全基因组水平上鉴定了苹果RR基因家族，共鉴定出61个MdRR基因。分析了MdRR基因的理化特性、染色体定位、基因结构、顺式调控元件、保守基序、系统进化分析及组织特异性表达模式。基因结构分析表明，同一亚族成员结构类似，不同亚族成员结构差异明显。顺式作用元件预测结果显示，MdRR启动子存在与光响应、植物激素与逆境胁迫相关的响应元件，如与细胞分裂素、生长素、干旱、低温等有关的响应元件。保守基序分析结果表明，不同成员之间具有差异性。系统进化分析将61个MdRR分为4个亚族，分别为ARR、BRR、CRR、PRR。组织特异性表达模式分析表明，MdRR成员在苹果不同组织部位中的表达水平差异较大。更多还原

【Abstract】 The RR gene family plays a crucial role in apple growth, and investigating the evolution and biological characteristics of MdRR genes is of significant importance. Using bioinformatics approaches, we identified the RR gene family at the genome-wide level in apple, comprising a total of 61 MdRR genes. We systematically analyzed their physicochemical properties, chromosomal localization, gene structure, cis-regulatory elements, conserved motifs, phylogenetic relationships, and tissue-specific expression patterns. Gene structure analysis revealed that members within the same subfamily exhibit similar exon-intron architectures, whereas distinct structural variations were observed among different subfamilies. Cis-element prediction demonstrated that MdRR promoters contain various stress and hormone responsive elements, including those associated with light response, cytokinin, auxin, drought, and low-temperature stress. Conserved motif analysis indicated considerable divergence among different MdRR members. Phylogenetic analysis classified the 61 MdRR genes into four subfamilies: ARR, BRR, CRR, and PRR. Furthermore, tissue-specific expression profiling revealed substantial variations in MdRR transcript levels across different apple tissues.更多还原