【摘要】 硒元素是人类必须的微量元素之一，其安全、高效生物转化载体的开发具有重要的研究价值。基于富硒酵母的生物安全性优势，通过耐硒和富硒能力筛选实验，获得一株富硒能力较强的菌株1769杰丁塞伯林德纳氏酵母。采用单因素及正交实验优化发酵工艺参数，重点研究了接种量、亚硒酸钠添加量、发酵时间等因素对菌体生物量及有机硒转化率的影响。结果表明，当接种量为5%、亚硒酸钠添加量为20 μg/mL、亚硒酸钠添加时间为8 h、发酵时间为24 h时，菌株1769发酵性能最佳，其富硒量为5 395.3 μg/g，有机硒转化率为93.25%，生物量为3.46 g/L。本研究可为富硒产品开发提供优质菌种资源，所建立的发酵工艺参数体系对工业化生产具有指导意义。更多还原

【Abstract】 Selenium is one of the essential trace elements for human beings,and the development of safe and efficient biotransformation carriers for it has important research value. In this study,based on the biosafety advantages of selenium-enriched yeast, a strain 1769(Cyberlindnera jadinii) with strong selenium-enrichment capacity was obtained through selenium tolerance and selenium-enrichment capacity screening experiments;One-way and orthogonal tests were used to optimize the fermentation process parameters,focusing on the effects of inoculum amount,sodium selenite addition fermentation time and other factors on the biomass and selenium conversion rate. The results showed that when the inoculum amount was 5%,the sodium selenite addition amount was 20 μg/mL,the sodium selenite addition time was 8 h,and the fermentation time was 24 h,the strain 1769 had the best fermentation performance,with the selenium-enrichment amount of 5 395.34 μg/g,the conversion rate of 93.25%,and the biomass of 3.46 g/L.This research not only provides high-quality strain resources for the development of selenium-enriched products but also establishes a system of fermentation process parameters which is of guiding significance for industrial production.更多还原