【摘要】 目的 观察油茶皂苷体外对白色念珠菌生物膜形成的影响，并探究其抗真菌的作用机制。方法 通过微量浓度稀释法测定油茶皂苷对白色念珠菌的最小抑菌浓度及对生物膜生长过程中不同时期的作用效果；用倒置显微镜观察油茶皂苷对白色念珠菌生物膜形成的影响，用实时荧光定量PCR对白色念珠菌生物膜相关基因als1、als3、hwp1的表达进行检测并比较差异化。结果 微量浓度稀释法显示，油茶皂苷抑制白色念珠菌酵母态50%活性的最低药物浓度为64μg/mL,对白色念珠菌各时期生物膜也有一定的抑制效果，并随生物膜时期和作用时间变化而有所差异；油茶皂苷实验组的白色念珠菌生物膜相关基因als1、als3、hwp1的表达较阴性对照组明显降低(P<0.05)。结论 油茶皂苷可能通过下调白色念珠菌生物膜相关基因als1、als3、hwp1的表达来抑制生物膜的形成，从而抵抗白色念珠菌的致病性。更多还原

【Abstract】 Objective To observe the effect of Sasanquasaponin on biofilm formation of C.albicans(Candida albicans) in vitro, and explore its antifungal mechanism.Methods Determine the minimum inhibitory concentration of Sasanquasaponin against C.albicans and their effects on biofilm growth at different stages using the micro concentration dilution method; Observe the effect of Sasanquasaponin on the formation of C.albicans biofilm using an inverted microscope, and then use real-time fluorescence quantitative PCR to detect the expression of C.albicans biofilm related genes als1,als3,and hwp1 and compare the differences.Results The micro concentration dilution method showed that the minimum drug concentration of Sasanquasaponin to inhibit 50% yeast activity of C.albicans was 64 μg/ml, and they also had a certain inhibitory effect on the biofilm of C.albicans at different stages, which varied with the biofilm period and action time; The expression of C.albicans biofilm related genes als1,als3,and hwp1 in the experimental group of Sasanquasaponin was significantly reduced compared to the negative control group(P<0.05).Conclusion Sasanquasaponin might inhibit the formation of C.albicans biofilms by downregulating the expression of genes related to biofilms such as als1,als3,and hwp1,thereby resisting the pathogenicity of C.albicans.更多还原