【摘要】 目的 探讨孕期六氟环氧丙烷二聚羧酸（HFPO-DA）暴露对母鼠及其胎鼠肝脏损伤的影响。方法 建立孕期大鼠HFPO-DA暴露模型，将孕0.5 d的SD大鼠随机分为空白对照组（给予去离子水）和不同浓度HFPO-DA暴露组（分别给予1 mg/kg、10 mg/kg、100 mg/kg的HFPO-DA）。实验组通过灌胃暴露至孕19.5 d，随后处死孕鼠。记录孕鼠及胎鼠的体质量和肝脏质量，采用苏木精–伊红染色（H-E染色）观察肝脏组织病理改变；全自动生化分析仪用于测定孕鼠血清中天冬氨酸氨基转移酶（AST）、碱性磷酸酶（ALP）、丙氨酸氨基转移酶（ALT）、总胆固醇（THO）、高密度脂蛋白胆固醇（HDL-C）水平、白蛋白（ALB）、总甘油三酯（TG）、总胆汁酸（TBA）和低密度脂蛋白胆固醇（LDL-C）。结果 与对照组相比，高剂量HFPO-DA暴露组孕鼠的肝脏质量和肝脏系数显著增加。H-E组织染色显示该组孕鼠肝脏汇管区充血、肝细胞点状坏死及肝细胞水肿。高剂量HFPO-DA组孕鼠血清中ALT、TBA、LDL-C水平显著高于对照组；此外，中剂量和高剂量组的HDL-C水平均显著低于对照组。胎鼠暴露组与对照组相比，肝脏质量及病理组织未见明显异常。结论 孕期HFPO-DA暴露可导致SD大鼠母鼠肝脏损伤及代谢指标异常，但对胎鼠的影响较小。更多还原

【Abstract】 Objective To investigate the effects of hexafluoropropylene oxide dimer acid(HFPO-DA) exposure during pregnancy on liver damage in maternal rats and their fetuses. Methods A rat model of HFPO-DA exposure during pregnancy was established. The pregnant Sprague-Dawley(SD) rats were randomly allocated to four groups on day 0.5 of gestation: A control group(administered deionized water) and three HFPO-DA exposure groups(receiving HFPO-DA via gavage at doses of 1 mg/kg, 10 mg/kg, and 100 mg/kg). All treatment groups underwent continuous gavage administration until day 19.5 of gestation, after which the pregnant rats were euthanized. Maternal and fetal body weights and liver weights were taken. Hematoxylin and eosin(H-E) staining was used to observe liver histopathological changes. Aspartate transferase(AST), alkaline phosphatase(ALP), Alanine amino–transferase(ALT), total cholesterol(THO), high-density lipoprotein cholesterol(HDL-C), albumin(ALB), triglycerides(TG), total bile acids(TBA), low-density lipoprotein cholesterol(LDL-C) were measured using an automated biochemical analyzer. Results Compared to the control group, the high-dose HFPO-DA group showed a significant increase in liver weight and organ coefficient in maternal rats. H-E tissue staining revealed congestion in the periportal areas, focal necrosis of hepatocytes, and hepatocellular edema in the high-dose group. The levels of ALT, TBA, and LDL-C in serum were significantly higher in the high-dose HFPO-DA group compared to the control group. Furthermore, HDL-C levels in the middle and high-dose groups were significantly lower than control group. No significant changes were observed in the liver weight or histopathological changes in the fetal rats exposed to HFPO-DA. Conclusion Exposure to HFPO-DA during pregnancy can cause liver damage and metabolic abnormalities in maternal rats, but has a relatively small effect on fetal rats.更多还原