【摘要】 目的：建立锌粉还原衍生、高效液相色谱-荧光检测法（HPLC-FLD）测定辛芩颗粒中马兜铃酸I(AA-I）的分析方法。方法：采用超声波法提取样品后，在酸性条件下用锌粉将提取液中无荧光的AA-I还原衍生成具有荧光的马兜铃内酰胺I(AL-I），再通过比较衍生前后HPLC-FLD检测结果之差求得AA-I的含量。结果：最佳衍生反应条件为：酸性条件下，室温反应15 min。在最佳条件下，AA-I在0.001～0.400μg/m L范围内线性关系良好，R2为0.993 6，低、中、高3个浓度基质加标的相对回收率在91.9%～107.8%之间，RSD小于8.1%。采用该方法测得3批次辛芩颗粒样品中AA-I的平均含量在0.59～0.66μg/g之间。结论：本研究建立的方法灵敏、快速，可为中药制剂中AA-I含量的准确测定提供参考。更多还原

【Abstract】 Objective:To establish a method for the determination of aristolochic acid I (AA-I) in Xinqin granules by zinc powder reduction derivatization and high-performance liquid chromatographyfluorescence detection (HPLC-FLD).Methods:After extracting the samples using the ultrasonic method,the extract was subjected to a reduction-derivatization process under acidic conditions with zinc powder to convert non-fluorescent AA-I into fluorescent aristololactam I (AL-I).By comparing the differences in HPLC-FLD detection results before and after derivatization,the content of AA-I could be determined.Results:The optimal derivatization reaction conditions were established as follows:the reaction was conducted at room temperature for 15 min under acidic conditions.Under optimal conditions,the method demonstrated a broad linear range of 0.001 to 0.400μg/m L,with a correlation coefficient (R2）of 0.993 6.The relative recoveries for matrix spiking experiments at low,medium,and high concentrations ranged from 91.9%to 107.8%,with a relative standard deviation(RSD) of less than 8.1%.The method was applied to determine the AA-I content in three batches of Xinqin granules,with the average content ranging from 0.59 to 0.66μg/g.Conclusion:The method established in this study was found to be highly sensitive and rapid,serving as a reference for the accurate determination of AA-I content in traditional Chinese medicinal preparations.更多还原