【摘要】 目的：运用数据挖掘与网络药理学技术总结分析贺菊乔教授治疗湿热瘀阻型慢性前列腺炎的用药规律，预测核心中药组合治疗湿热瘀阻型慢性前列腺炎的潜在作用机制。方法：收集贺菊乔教授于2023年5月15日-2023年6月14日门诊收治的42诊次湿热瘀阻型慢性前列腺炎患者的病历资料，对其用药规律进行数据挖掘分析，利用网络药理学技术探究核心中药组合治疗湿热瘀阻型慢性前列腺炎的潜在作用机制。结果：共收集到42诊次湿热瘀阻型慢性前列腺炎患者，共涉及93味中药。频次统计分析发现，贺菊乔教授治疗湿热瘀阻型慢性前列腺炎用药性味以寒、甘为主，其中使用寒性中药频次占比为33.70%，甘味中药频次占比为33.11%；中药使用频次较高的依次为白花蛇舌草(33次)、败酱草(33次)、丹参(28次)，由此可知，贺老治疗湿热瘀阻型慢性前列腺炎的常用中药组合为白花蛇舌草–败酱草–丹参。通过网络药理学分析获得白花蛇舌草–败酱草–丹参组合的活性成分57个，潜在作用靶点138个。通过蛋白质–蛋白质相互作用网络分析获得9个核心靶点，包括信号转导和转录激活因子3(Signal Transducer and Activator of Transcription 3,STAT3)、肿瘤蛋白p53(Tumor Protein p53,TP53)、丝氨酸/苏氨酸蛋白激酶1(Akt Serine/Threonine Kinase 1,AKT1)等。通过京都基因与基因组百科全书(KEGG)通路富集分析发现，核心中药组合可通过磷脂酰肌醇3激酶(Phosphatidylinositol 3–kinase,PI3K)–蛋白激酶B(Akt)、晚期糖基化终末产物(Advanced Glycation End Product,AGE)–晚期糖基化终末产物受体(Advanced Glycation End Product Receptor,RAGE)、肿瘤坏死因子(Tumor Necrosis Factor,TNF)、白细胞介素–6(Interleukin–6,IL–6)等信号通路作用于湿热瘀阻型慢性前列腺炎，并与前列腺癌、癌症中的蛋白聚糖、细胞凋亡等途径相关。结论：贺菊乔教授治疗湿热瘀阻型慢性前列腺炎用药以清热解毒药为主，其处方用药规律符合这一辨证思路，核心中药组合为白花蛇舌草–败酱草–丹参，其主要成分为槲皮素、木犀草素，作用于STAT3、细胞周期蛋白D1(Cyclin D1,CCND1)、丝裂原活化蛋白激酶(Mitogen–Activated Protein Kinase,MAPK)1等靶点，通过PI3K–Akt、AGE–RAGE、TNF与癌症相关信号通路等途径发挥治疗湿热瘀阻型慢性前列腺炎的作用。更多还原

【Abstract】 Objective: To systematically summarize and analyze the medication rules of Professor HE Juqiao in treating chronic prostatitis of damp-heat and blood stasis type using data mining and network pharmacology, and to predict the potential action mechanisms of the core herbal combination for this condition. Methods: Medical records of 42 patients with chronic prostatitis of damp-heat and blood stasis type treated by Professor HE Juqiao in his outpatient clinic from May 15, 2023 to June 14, 2023 were collected. Data mining was performed to analyze medication patterns, and network pharmacology was employed to explore the underlying pharmacological mechanisms of the core herbal combination. Results: Among the 42 cases of chronic prostatitis of damp-heat and blood stasis type, a total of 93 herbs were utilized. Frequency analysis revealed that the predominant natures of the herbs used by Professor HE Juqiao were cold in nature(33.7%) and sweet in flavor(33.11%). The most frequently prescribed herbs were Baihua She She Cao(Herba Hedyotis Diffusae)(33 times), Bai Jiang Cao(Herba Patriniae)(33 times), and Dan Shen(Radix et Rhizoma Salviae Miltiorrhizae)(28 times). This indicates that the common herbal combination used by Professor HE is Baihua She She Cao-Bai Jiang Cao-Dan Shen. Network pharmacology analysis identified 57 active components and 138 potential targets associated with this combination. Protein-protein interaction(PPI) network analysis further revealed 9 core targets, including STAT3, TP53, and AKT1. Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis demonstrated that the core herbal combination may exert its therapeutic effects on chronic prostatitis of damp-heat and blood stasis type through pathways such as PI3K-Akt, AGE-RAGE, TNF and IL-6, which are also related to prostate cancer, proteoglycans in cancer, and apoptosis. Conclusion: Professor HE Juqiao’s treatment strategy for chronic prostatitis of damp-heat and blood stasis type primarily involves heat-clearing and detoxifying herbs and is consistent with the principles of traditional Chinese medicine. The core herbal combination of Baihua She She Cao-Bai Jiang Cao-Dan Shen contains major bioactive components such as quercetin and luteolin, which act on key targets like STAT3, CCND1, and MAPK1. These components modulate signaling pathways including PI3K-Akt, AGE-RAGE, TNF, and cancer-related pathways, thereby contributing to the therapeutic effects observed in chronic prostatitis of damp-heat and blood stasis type.更多还原