【摘要】 目的 以高效液相色谱-电感耦合等离子体质谱（highperformanceliquid chromatography-inductively coupled plasma mass spectrometry,HPLC-ICP-MS）法为检测手段，建立同时测定工作场所空气中三价铬Cr（Ⅲ）和六价铬Cr（Ⅵ）含量的方法。方法 样品用碱性聚偏二氟乙烯（polyvinylidene difluoride,PVDF）滤膜采集，通过乙二胺四乙酸（ethylene diamine tetraacetic acid,EDTA）-硝酸铵溶液洗脱，以硝酸铵为流动相，液相色谱分离，电感耦合等离子体质谱检测，外标法定量。结果 三价铬和六价铬在1～500μg/L范围内相关系数>0.999，方法检出限分别为0.000 024 mg/m~3和0.000 034 mg/m~3（以采样45 L计算），定量限为0.000 44 mg/m~3；加标回收率在87.7%～105%之间，相对标准偏差均<5%。结论 该方法准确、灵敏、检测范围宽，适用于工作场所空气中Cr（Ⅲ）和Cr（Ⅵ）的同时测定，为职业安全检测领域的发展提供了参考。更多还原

【Abstract】 Objective To establish a method for the simultaneous determination of trivalent chromium[Cr （Ⅲ ）] and hexavalent chromium[Cr（Ⅵ）] in workplace air using high-performance liquid chromatography-inductively coupled plasma mass spectrometry（HPLC-ICP-MS）. Methods Samples were collected using an alkaline polyvinylidene difluoride（PVDF） filter,eluted with an ethylene diamine tetraacetic acid（EDTA）-NH₄NO₃solution,separated by liquid chromatography using NH₄NO₃as the mobile phase,and detected by inductively coupled plasma mass spectrometry. Quantification was performed using an external standard calibration curve. Results The correlation coefficients for Cr（Ⅲ ） and Cr（Ⅵ） were greater than 0.999 within the concentration range of 1-500 μg/L. The limits of detection were 0.000 024 mg/m³and 0.000 034 mg/m³,respectively（based on a sampling volume of 45 L）,and the quantification limit was 0.000 44 mg/m³. The recovery rate ranged from 87.7% to 105%,with relative standard deviations all below 5%. Conclusion This method is accurate,sensitive,and has a wide detection range,it is suitable for the simultaneous determination of Cr（Ⅲ ） and Cr（Ⅵ） in workplace air,providing a reference for the advancement of occupational safety testing.更多还原