【摘要】 目的 分析浙江省温州市龙湾区食源性疾病主动监测样本分离的沙门菌血清型和耐药性，为沙门菌感染防治提供依据。方法 收集2018—2024年龙湾区人民医院就诊的食源性腹泻患者粪便或肛拭子样本中分离的沙门菌，复核鉴定后采用玻片凝集试验鉴定血清型；采用微量肉汤稀释法对沙门菌活菌进行药敏试验，分析耐药性。结果 收集样本2 293份，检出沙门菌186株，总检出率为8.11%,5—10月检出率较高。确定沙门菌血清型28种，其中鼠伤寒沙门菌、肠炎沙门菌和伦敦沙门菌分别为72、31和30株，占38.71%、16.67%和16.13%。121株沙门菌活菌中，20株对14种抗菌药物均敏感；101株对部分抗菌药物耐药，耐药率为1.65%～67.77%，其中氨苄西林耐药率最高，亚胺培南耐药率最低；鼠伤寒沙门菌对四环素耐药率最高，为78.26%；肠炎沙门菌对氨苄西林耐药率最高，为100.00%；伦敦沙门菌对四环素耐药率最高，为66.67%。检出55种耐药谱型，呈现1～10重耐药，其中多重耐药菌株76株，占75.25%；多重耐药谱型主要为耐氨苄西林/舒巴坦-头孢唑林-氨苄西林-萘啶酸、耐四环素-氨苄西林-萘啶酸和耐氨苄西林/舒巴坦-氨苄西林-萘啶酸，分别占10.53%、9.21%和7.89%。结论 龙湾区食源性疾病沙门菌主要在夏秋季检出，血清型分布广泛，以鼠伤寒沙门菌、肠炎沙门菌和伦敦沙门菌为主；沙门菌对不同抗菌药物的耐药性不同，耐药谱型呈现多样性。更多还原

【Abstract】 ObjectiveTo analyze the serotypes and drug resistance of Salmonella isolated from food-borne disease surveillance samples in Longwan District, Wenzhou City, Zhejiang Province, so as to provide evidence for the prevention and treatment of Salmonella infection.Methods Salmonellastrains isolated from feces or anal swabs of patients with foodborne diarrhea in Longwan District People’s Hospital from 2018 to 2024 were collected. After re-identification, slide agglutination test was used to identify serotypes. The drug susceptibility test of live Salmonella strains was performed using the broth microdilution method, and the resistance patterns were analyzed.ResultsA total of 2 293 samples were collected, and 186 strains of Salmonella were isolated, with a detection rate of 8.11%. The detection rate was higher from May to October. A total of 28 Salmonella serotypes were identified, with S. typhimurium(72 isolates, 38.71%),S. enteritidis(31 isolates, 16.67%), and S. London(30 isolates, 16.13%) being dominant. Among the 121 Salmonella live strains, 20 strains were susceptible to 14 antibacterial drugs. A total of 101 strains were resistant to antibacterial drugs, and the drug resistance rate was 1.65%-67.77%, with the drug resistance rate of ampicillin being the highest,and the drug resistance rate of imipenem was the lowest. S. typhimurium had the highest resistance rate to tetracycline(78.26%). S. enteritidis had the highest resistance rate to ampicillin(100.00%). S. London had the highest resistance rate to tetracycline(66.67%). Fifty-five types of drug resistance patterns were detected, showing a number of drug resistance of 1-10, of which 76 strains were multi-drug resistant, accounting for 75.25%. The predominant multidrug resistance patterns were ampicillin/sulbactam-cefazolin-ampicillin-nalidixic acid(10.53%), tetracycline-ampicillin-nalidixic acid(9.21%), and ampicillin/sulbactam-ampicillin-nalidixic acid(7.89%).Conclusions Salmonellastrains isolated from foodborne diseases in Longwan District were mainly detected in summer and autumn. S. typhimurium, S. enteritidis, and S. London were the predominant serotypes. The drug resistance of Salmonella to different antibacterial drugs was different, and the drug resistance spectrum showed diversity.更多还原