【摘要】 目的:建立HPLC一测多评法（quantitative analysis of multi-components by single marker, QAMS）同时测定蒙药孟根·沃斯-18丸中没食子酸、表儿茶素、鞣花酸、杨梅素、肉桂酸、丁香酚、橙黄决明素、甘松新酮、α-细辛脑和大黄酚等10种成分，并验证该方法在蒙药孟根·沃斯-18丸质量分析中应用的可行性。方法:采用Agilent Eclipse Plus C₁₈色谱柱（250 mm×4.6 mm, 5μm）,流动相为乙腈（A）-0.2%磷酸溶液（B）,梯度洗脱（0～60 min, 5%A→25%A,波长210 nm; 60～80 min, 25%A→36%A,波长280 nm; 80～145 min, 36%A→75%A,波长250 nm）,流速为1.0 mL·min^-1;柱温为40℃。以鞣花酸为内参物，建立与其他9种待测成分的相对校正因子(f_s/i),采用f_s/i计算各成分的质量分数，同时用外标法（external standard method, ESM）测定，比较2种方法测定结果，评价QAMS在蒙药孟根·沃斯-18丸中应用的准确性。结果:蒙药孟根·沃斯-18丸中没食子酸、表儿茶素、杨梅素、肉桂酸、丁香酚、橙黄决明素、甘松新酮、α-细辛脑和大黄酚9种成分的f_s/i重现性良好，分别为0.242 2,0.231 5,0.319 9,0.256 5,1.969 9,1.208 9,1.260 2,0.560 5和1.213 2,QAMS,ESM所得结果接近。结论:该方法简便、稳定、重复性良好，可用于孟根·沃斯-18丸的质量控制。更多还原

【Abstract】 Objective: To establish an HPLC method for simultaneous determination of 10 components（gallic acid, epicatechin, ellagic acid, myricetin, cinnamic acid, eugenol, hesperidin, alpha-asarol and chrysophanol） in a Mongolian medicine, Menggen Vos-18 pills, and verify the feasibility of applying this method in its quality analysis. Methods: The chromatography was performed on Agilent Eclipse Plus C₁₈ column（250 mm×4.6 mm, 5 μm） with mobile phase consisting of acetonitrile（A）-0.2% phosphoric acid solution（B） and gradient elution（0～60 min, 5%A→25%A, wavelength 210 nm; 60～80 min, 25%A→36%A, wavelength 280 nm; 80～145 min, 36%A→75%A, wavelength 254 nm）, and the flow rate was 1.0 mL·min^-1. The column temperature was 40 ℃. Relative correction factors(f_s/i) were established with ellagic acid as the internal reference, the mass fractions of each component were calculated by relative correction factor, and the results were determined by external standard method. The accuracy of the application of quantitative analysis of multi-components by single marker（QAMS） method in Menggen Vos-18 pills was evaluated. Results: The f_s/i reproducibility of gallic acid, epicatechin, myricetin, cinnamic acid, eugenol, hesperidin, manpinone, alpha-asarol and chrysophenol in Menggen Vos-18 pills was good. They were 0.242 2, 0.231 5, 0.319 9, 0.256 5, 1.969 9, 1.208 9, 1.260 2, 0.560 5 and 1.213 2, respectively. The results obtained by QAMS method and external standard method were close to each other. Conclusion: The method is simple, stable and reproducible, and can be used for the quality control of Mengen Vos-18 pills.更多还原