【摘要】 目的:探究鸡骨草水提物对鸭乙型肝炎病毒(DHBV)模型的抗病毒作用及其护肝效果。方法:通过腹腔注射建立DHBV感染模型后，随机抽取感染阳性的50只麻鸭，分为阳性对照组、模型组和鸡骨草水提物高、中、低剂量组，每组10只；随机抽取感染阴性的10只麻鸭作为正常对照组。阳性对照组麻鸭给予拉米夫定，鸡骨草水提物高、中、低剂量组分别按1.604、0.802、0.401 g/kg剂量鸡骨草水提物灌胃，模型组和正常对照组灌服纯水，1次/d,持续给药14 d。在给药前(T0)、给药后第7天(T7)、给药后第14天(T14)、停药后第5天(P5)时收取麻鸭血清样本。采用实时荧光定量(RT-PCR)技术检测DHBV DNA水平，采用酶联免疫吸附分析(ELISA)检测血清中DHBV表面抗原(HBsAg)和e抗原(HBeAg)的含量，并用试剂盒检测血清丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)水平。采集麻鸭肝脏组织样本，经苏木精-伊红染色后观察其病理改变。结果:阳性对照组麻鸭T14时DHBV DNA明显降低(P<0.05);未发现鸡骨草水提物存在抑制病毒载量的效果，未观察到抑制HBsAg、HBeAg的作用(P>0.05);鸡骨草水提物有一定的降低ALT水平的作用(P<0.05),但未发现鸡骨草水提物有降低AST水平的作用(P>0.05);高剂量的鸡骨草水提物能有效减低肝脏病理损害程度(P<0.05)。结论:未发现鸡骨草水提物有抗DHBV效果，但有一定的抗肝损伤作用。更多还原

【Abstract】 Objective:To investigate the hepatitis B virus(HBV) model duck’ anti-HBV effect and liver protection of water extract of aqueous extract of Abrus Cantoniensis Hance.Methods:After establishing a DHBV infection model via intraperitoneal injection, 50 infected Muscovy ducks were randomly selected and divided into positive control group, model group, and high, medium, and low dose groups of the aqueous extract of Abrus Cantoniensis Hance, with 10 ducks in each group. Another 10 uninfected Muscovy ducks were randomly selected as the normal control group. The positive control group of ducks was administered lamivudine, while the high, medium, and low dose groups of the aqueous extract of Abrus cantoniensis Hance were given 1.604, 0.802, and 0.401 g/kg of the extract respectively by gavage. The model and normal control groups were given pure water by gavage, once a day for 14 days. Serum samples from the ducks were collected before treatment(T0), on day 7 after treatment(T7), on day 14 after treatment(T14), and on day 5 after discontinuation of treatment(P5). Real-time quantitative PCR(RT-PCR) was used to detect the level of DHBV DNA, and enzyme-linked immunosorbent assay(ELISA) was used to measure the levels of DHBV surface antigen(HBsAg) and e antigen(HBeAg) in the serum. The levels of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) in the serum were detected using a kit. Liver tissue samples from the ducks were collected, stained with hematoxylin and eosin, and examined for pathological changes.Results:Viral load was significantly lower in the positive control group on T14(P<0.05). No effect of viral load suppression was found in the water extract of Abrus Herb, and no reduction of HBsAg and HBeAg was observed(P>0.05).The low dose of water extract of Abrus Herb could decrease ALT level to some extent(P<0.05), but no effect of lowering the AST level was found(P>0.05). High dose of water extract of Abrus Herb can reduce the degree of liver pathological damage(P<0.05).Conclusion:Water extract of Abrus Herb has not been found to have anti-DHBV effect, but it has certain anti-liver injury effect.更多还原