【摘要】 甜菜（Beta vulgaris）是中国主要糖料作物之一,属中度耐盐植物。H⁺-ATPase在植物盐胁迫响应中发挥重要作用,但其在糖料作物中的耐盐功能研究较为匮乏。为研究BvATPase4基因在甜菜中的耐盐功能,本研究基于转录组数据分离出该基因,进行生物信息学分析,并成功转入拟南芥中分析其在植物响应盐胁迫过程中发挥的作用。结果表明BvATPase4在叶中表达量最高,且被200 mmol·L^-1 NaCl大量诱导表达。在盐胁迫下,转基因拟南芥的萌发率、存活率、根长、鲜重和叶绿素含量显著高于野生型;转基因拟南芥的Na⁺/K+较野生型更稳定,且超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）的活性以及可溶性糖、可溶性蛋白、游离脯氨酸（Pro）含量显著增高,同时丙二醛（MDA）、超氧阴离子(O₂^·-)和过氧化氢（H₂O₂）含量显著降低,表明BvATPase4能通过维持离子稳态、改善渗透调节和抗氧化调节来提升转基因植物对盐胁迫的耐受性。以上结果初步说明BvATPase4基因在甜菜中的耐盐功能,为甜菜耐盐性遗传改良提供重要候选基因。更多还原

【Abstract】 Sugar beet（Beta vulgaris）, as one of the principal sugar crops in China, is a moderately salt-tolerant plant. H+-ATPase plays a crucial role in plant salt stress response, yet its salt-tolerant functions in sugar crops remain insufficiently investigated. To elucidate the salt-tolerant function of Bv ATPase4 gene in sugar beet, this study isolated the target gene based on transcriptome data, conducted bioinformatics analysis,and successfully generated transgenic Arabidopsis for functional characterization during salt stress response. Results demonstrated that Bv ATPase4 exhibited highest expression levels in leaves and was significantly induced by 200 mmol·L^-1 NaCl. Under salt stress, the germination rate, survival rate, root length,fresh weight, and chlorophyll content were significantly higher than those of the wild type. Transgenic plants maintained more stable Na+/K+ ratios and displayed enhanced activities of superoxide dismutase（SOD）,peroxidase（POD）, and catalase（CAT）, along with elevated accumulation of soluble sugars, soluble proteins,and free proline（Pro）. Concurrently, malondialdehyde（MDA）, superoxide anion(O₂^·-), and hydrogen peroxide（H₂O₂） contents were significantly reduced. These findings indicate that Bv ATPase4 enhances salt stress tolerance in transgenic plants through maintaining ionic homeostasis, improving osmotic regulation, and enhancing antioxidant capacity. This study preliminarily elucidates the salt-tolerant function of Bv ATPase4in sugar beet, providing a valuable candidate gene for genetic improvement of salt tolerance in this crop.更多还原