【摘要】 库尔勒香梨(Pyrus sinkiangensis)是新疆库尔勒地区的特色果树,对当地农业经济和农民生计具有重要影响。为应对极端干旱天气,本研究利用生物信息学分析PsHB7蛋白的理化性质、蛋白结构和进化关系,旨在探索PsHB7基因在提高转基因番茄耐旱性中的作用。结果显示, PsHB7蛋白具有高度保守的HD和HALZ结构域,属于HD-Zip I家族成员,与苹果(Malus domestica)的HB7蛋白的亲缘关系最近。利用分子生物学技术,我们克隆获得PsHB7基因,其开放阅读框(ORF)长度为729 bp,编码242个氨基酸。构建35S::PsHB7过表达载体,并通过农杆菌介导的遗传转化方法将其转入番茄。在干旱胁迫下,转PsHB7基因番茄表现出显著的水分保持能力,叶片中的脯氨酸含量显著增加,膜脂过氧化水平显著降低。这表明PsHB7基因的过表达能够增强植物的干旱耐受性,为培育耐旱性强的库尔勒香梨新品种提供有效的分子策略。更多还原

【Abstract】 The Korla pear(Pyrus sinkiangensis) endemic to the Korla region of Xinjiang, plays a vital role in the local agricultural economy and the livelihoods of farmers. In response to extreme drought conditions,this study utilizes bioinformatics analysis to investigate the physicochemical properties, protein structure,and evolutionary relationships of the PsHB7 protein, aiming to explore the role of the PsHB7 gene in enhancing the drought tolerance of genetically modified tomatoes. Through bioinformatic analysis, we characterized the physicochemical properties, structural features, and phylogenetic relationships of the PsHB7protein, identifying it as a conserved member of the HD-Zip I family, with its closest homology to the HB7protein of apple(Malus domestica). The PsHB7 gene was cloned utilizing molecular biology techniques,featuring a 729 bp open reading frame that encodes a 242 amino acid. We then constructed a 35S::PsHB7overexpression vector and transformed it into tomatoes via Agrobacterium-mediated transformation. The transgenic tomatoes demonstrated superior water retention under drought stress, accompanied by increased leaf proline content and reduced membrane lipid peroxidation. These findings indicate that PsHB7overexpression can enhance plant drought tolerance, offering a promising molecular strategy for developing Pyrus sinkiangensis cultivars with improved drought tolerance.更多还原