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河南省花生白绢病菌的菌丝亲和群及对丙硫菌唑的敏感性分析

Mycelial compatibility groups of Sclerotium rolfsii from peanut and its sensitivity to prothioconazole in Henan province

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【作者】 吕雪; 胡艳红; 徐建强; 康业斌; 崔林开;

【Author】 L Xue;HU Yanhong;XU Jianqiang;KANG Yebin;CUI Linkai;College of Horticulture and Plant Protection, Henan University of Science and Technology;Henan Province Engineering Technology Research Center of Green Plant Protection;

【通讯作者】 崔林开;

【机构】 河南科技大学园艺与植物保护学院; 河南省绿色植保工程技术研究中心;

【摘要】 由齐整小核菌Sclerotium rolfsii引起的花生白绢病是花生上一种毁灭性的土传病害。为明确河南省花生白绢病菌的菌丝亲和群(mycelial compatibility groups, MCGs)以及对丙硫菌唑的敏感性,本研究从河南省安阳、开封、驻马店和南阳4个地区分离花生白绢病菌109株,分别采用配对培养法和菌丝生长速率法进行菌丝亲和群分析和对丙硫菌唑的敏感性测定。结果表明,109株花生白绢病菌共划分出10个MCGs,其中MCG1和MCG2的菌株数量为70株和27株,两者占比达88.99%,为优势MCGs。丙硫菌唑对河南省花生白绢病菌的EC50范围在0.041 7~2.182 9 mg/L之间,平均值为(0.450 7±0.471 9) mg/L。不同地区菌株对丙硫菌唑的敏感性存在显著差异,驻马店地区菌株的EC50最高,平均值为(0.626 3±0.606 0) mg/L,南阳地区菌株的EC50最低,平均值为(0.312 8±0.424 5) mg/L。2个优势菌丝亲和群MCG1和MCG2对丙硫菌唑的敏感性没有显著差异。MCG1和MCG2菌株的平均EC50分别为(0.385 1±0.381 9) mg/L和(0.379 7±0.361 0) mg/L。这些研究结果为制定河南省花生白绢病高效防治策略提供了理论依据。

【Abstract】 Peanut stem rot, caused by Sclerotium rolfsii, is a devastating soil-borne disease that threatens peanut production. To clarify the distribution of mycelial compatibility groups(MCGs) of S. rolfsii and their sensitivity to prothioconazole in Henan province, 109 isolates were collected from Anyang, Kaifeng, Zhumadian, and Nanyang. MCGs were determined using the confrontation culture method, and sensitivity to prothioconazole was examined by using the mycelial growth rate method. A total of 10 MCGs were identified among the isolates. MCG1 and MCG2 contained 70 and 27 isolates, respectively, counting for 88.99% of the total and representing the dominant MCGs. The EC50values of S.rolfsii isolates to prothioconazole ranged from 0.041 7 mg/L to 2.182 9 mg/L, with a mean of(0.450 7±0.471 9) mg/L. Significant regional differences in sensitivity were observed: isolates from Zhumadian exhibited the highest mean EC50 value [(0.626 3±0.606 0) mg/L], whereas isolates from Nanyang had the lowest [(0.312 8±0.424 5) mg/L]. No significant difference in sensitivity to prothioconazole was detected between MCG1 and MCG2, with mean EC50 values of(0.385 1±0.381 9) mg/L and(0.379 7±0.361 0) mg/L, respectively. These findings provide a theoretical basis for developing effective chemical control strategies against peanut stem rot in Henan province.

【基金】 河南省重大科技专项(221100110100);河南省科技攻关项目(212102110141)
  • 【分类号】S435.652
  • 【下载频次】74
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