【摘要】 为了初步鉴定吉林省草莓病毒种类，本文以吉林省双阳、长白、梨树、公主岭4个县(区)的40个草莓样本为试验材料，通过宏病毒组测序和RT-PCR技术进行检测，结果共检测出草莓病毒1(StrV-1)、草莓轻型黄边病毒(SMYEV)和草莓镶脉病毒(SVBV)3种病毒。通过基因序列比对发现，StrV-1 L基因与捷克分离物C(MK211272.1)序列相似性最高，为77.85%,SMYEV CP基因与北京分离物BJHYZ83(OQ995373.1)序列相似性最高，为98.90%,SVBV CP基因与北京分离物BJHYZ1(MT027006.1)序列相似性最高，为99.64%。本研究对吉林省主产区的草莓病毒病进行了初步检测鉴定，优化了SMYEV和StrV-1吉林分离株的RT-PCR检测方法，而且以大肠杆菌BL21(DE3)表达的SMYEV衣壳蛋白为免疫原制备出2个能够识别SMYEV的单克隆抗体4G2、5G2,为吉林省草莓病毒病快速检测鉴定及防控提供了技术和材料基础。更多还原

【Abstract】 To preliminarily clarify the virus species infecting strawberry in Jilin province, 40 samples from Shuangyang, Changbai, Lishu, Gongzhuling were tested using metaviromes sequencing and RT-PCR. Three viruses were detected: strawberry virus 1(StrV-1), strawberry mild yellow edge virus(SMYEV) and strawberry vein banding virus(SVBV). Pairwise sequence comparison showed that the StrV-1 L gene shared the highest sequence similarity(77.85%) with Czech isolate C(MK211272.1), the SMYEV CP gene shared 98.90% the highest sequence similarity with Beijing isolate BJHYZ83(OQ995373.1), and the SVBV CP gene shared 99.64% the highest sequence similarity with Beijing isolate BJHYZ1(MT027006.1). This study provided a preliminary detection and identification of strawberry viruses in the main producing areas of Jilin province and optimized RT-PCR assays for the Jilin isolates of SMYEV and StrV-1. In addition, two monoclonal antibodies, 4G2 and 5G2, that recognize native SMYEV were prepared using SMYEV CP expressed in Escherichia coli BL21(DE3) as the immunogen. These results provide a technical and material basis for rapid detection, identification and control of strawberry virus diseases in Jilin province.更多还原