【摘要】 为揭示产黄青霉Penicillium chrysogenum Snef1216防治大豆胞囊线虫Heterodera glycines的作用机制，于室内测定产黄青霉Snef1216发酵液对大豆胞囊线虫卵和2龄幼虫的影响，染色观察接种产黄青霉Snef1216后大豆胞囊线虫对大豆的侵染情况和大豆根部木质素的变化，并测定产黄青霉Snef1216处理后大豆体内肉桂酰辅酶A还原酶（cinnamoyl-CoA reductase,CCR）基因表达量和大豆根部木质素含量。结果显示：产黄青霉Snef1216发酵原液对大豆胞囊线虫卵孵化有显著的抑制作用，处理48 h时对卵孵化的抑制率达89.11%；产黄青霉Snef1216发酵原液对大豆胞囊线虫2龄幼虫有显著的触杀作用，处理48 h时校正死亡率达94.30%。裂根染色试验显示产黄青霉Snef1216能有效抵御大豆胞囊线虫的侵染，产黄青霉Snef1216包衣处理大豆种子后其根内大豆胞囊线虫数量较对照显著减少了31.63%。产黄青霉Snef1216包衣后接种大豆胞囊线虫20 d时，大豆体内GmCCR相对表达量上调，为对照的2.09倍；木质素染色结果显示产黄青霉Snef1216发酵液包衣处理大豆种子能诱导木质素大量合成；产黄青霉Snef1216包衣后接种大豆胞囊线虫5 d时能诱导大豆根部木质素含量显著增加，为对照的1.71倍。表明大豆胞囊线虫胁迫下产黄青霉Snef1216通过诱导木质素合成抵御线虫侵染。更多还原

【Abstract】 To elucidate the mechanism by which Penicillium chrysogenum Snef1216 controls soybean cyst nematode Heterodera glycines, laboratory experiments were conducted to evaluate the effects of its fermentation broth on nematode eggs and second-stage juveniles(J2). Staining techniques were used to observe nematode infection and lignin deposition in soybean roots after Snef1216 treatment. Additionally, the expression level of the cinnamoyl-CoA reductase, cinnamoyl-CoA reductase(CCR) gene and lignin content in soybean roots were quantified. The results showed that the fermentation broth significantly inhibited nematode egg hatching, with an inhibition rate of 89.11% at 48 h. It also exhibited a strong contact-killing effect on J2s, with a corrected mortality rate of 94.30%. Root staining showed that Snef1216 effectively nematode infection, lowering nematode numbers in roots by 31.63% compared to controls. At 20 days post-inoculation, GmCCR expression in treated soybean plants was 2.09 times higher than in controls. Lignin staining confirmed strong induction of lignin accumulation in roots following seed coating with the Snef1216 fermentation broth, and lignin content at 5 days postinoculation was 1.71 times that of the control group. These results indicate that P. chrysogenum Snef1216 enhances soybean resistance to nematode infection by inducing lignin biosynthesis under nematode stress.更多还原