【摘要】 目的 探讨高含量、高脱乙酰度壳寡糖对非酒精性脂肪肝小鼠的辅助保护作用。方法 将75只KM小鼠随机分为5组：3个样品试验组、模型组、阴性对照组。样品试验组和模型组每日摄入高脂饲料，阴性对照组予以常规饲料连续8周。第9周开始连续30 d经口灌胃样品试验组低(0.125 g)、中(0.25 g)、高(0.75 g)剂量的壳寡糖，阴性对照组和模型对照组给予等量纯水。在第31天处死动物，采血清测肝功能，观察肝组织外观性状并进行组织病理学检查。同时取部分肝组织匀浆，测定TG、GSH、MDA、TNF-α、TGF-β、IL-6等含量的变化。结果 经口灌胃小鼠不同剂量的壳寡糖30 d后，中剂量组小鼠肝组织中的AST、TGF-β、TNF-α分别为(68.0±5.4)U/L、(62±5.0)pg/mL、(52±3.0)pg/mL,高剂量组肝组织中的TG(0.25±0.15)mmol/L、MDA(2.3±0.75) nmol/L、IL-6(2.8±0.3)pg/mL、ALT(35.5±2.7)U/L,模型组AST(89.5±9.4)U/L、TGF-β(75±4.2)pg/mL、TNF-α(60±5.3)pg/mL、TG(0.3±0.1)mmol/L(P<0.05);中、高剂量组GSH含量分别为(2.0±0.4)μmol/gprot和(2.1±0.8)μmol/gprot,较模型组的(1.5±0.9)μmol/gprot有所升高(P<0.05)。肝脏病理学检查结果表明：中、高剂量组小鼠体内肝脏中的脂滴分布范围小于模型组且面积积分低于模型对照组(P<0.05)。结论 高含量、高脱乙酰度壳寡糖对非酒精性脂肪肝有辅助保护作用。更多还原

【Abstract】 Objective To study the protective effect of chitosan oligosaccharides on mouse nonalcoholic fatty liver disease. Methods 75 KM mice were randomly divided into 5 groups. Three sample groups and the model group were given with a high-fat diet daily for 8 weeks, while the negative group was given ordinary diet consecutively. Three sample groups of mice were given different doses of samples by oral gavage for 30 days after nine weeks. The animals of the negative control group and the model control group were simultaneously given equal amounts of pure water. At the end of the experiment, the animals were weighed and fasted for 16 hours. On the 31st day, the animals were anesthetized and killed. Blood samples were collected. We performed anatomical observation and histopathology examination on the liver tissue. Simultaneously take some liver tissue homogenate and examine changes in levels of TG, GSH, MDA, TNF-α、TGF-β and IL-6 in it. Results After administering different doses of chitosan oligosaccharides to mice by oral gavage for 30 days,the level of AST, TGF-β and TNF-α(68.0±5.4 U/L, 62±5.0 pg/mL, 52±3.0 pg/mL)in liver tissue of medium dose group, and the level of TG(0.25±0.15 mmol/L), MDA(2.3±0.75 nmol/L), IL-6(2.8±0.3 pg/mL), ALT(35.5±2.7 U/L) in the liver tissue of high-dose groups are decreased(AST 89.5±9.4 U/L,TGF-β 75±4.2 pg/mL, TNF-α 60±5.3 pg/mL, TG 0.3±0.1 mmol/L)(P<0.05)compared with the model group, while the concentration of GSH in the medium and high-dose groups(Medium 2.0±0.4 μmol/gprot, High-dose 2.1±0.8 μmol/gprot) is increased(P<0.05), the results of liver pathology examination showed that the distribution, range, and area of lipid droplets in the medium and high-dose groups were lower than those in the model control group(P<0.05). Conclusion Chitosan oligosaccharides display an auxiliary protective effect on non-alcoholic fatty liver disease.更多还原