【摘要】 为了调查江西省鄱阳湖地区活禽市场低致病性禽流感病毒（LPAIV）的流行现状并评估LPAIV的公共卫生风险，本研究于2023年6月—2024年5月对鄱阳湖地区的都昌县活禽市场进行持续监测，采集811份样本（鸡源、鸭源和环境源），采用鸡胚分离病毒，通过病原核酸检测分析LPAIV的流行情况、不同月份LPAIV亚型的流行特点、LPAIV分离株的样本来源分布和不同样本来源LPAIV亚型的分布，并确定不同亚型LPAIV分离株的受体结合特性。结果显示，LPAIV总体检出率为53.4%(433/811)，其中H9亚型占比最高（27.5%,223/811），其次为H6亚型（9.4%,76/811）和H3亚型（1.6%,13/811），此外普遍存在不同亚型LPAIV的混合感染，并且H6和H9亚型LPAIV每月均有检出。从LPAIV分离株的样本来源分析，环境源样本的LPAIV检出率最高（68.9%），其次为鸡源（60.2%）和鸭源（41.7%）；从LPAIV亚型多样性分析，鸡源分离株以H9亚型为主，鸭源和环境源分离株亚型多样性较丰富（以H3和H6亚型为主，且含多亚型混合感染）。受体结合特性分析结果显示，H9亚型LPAIV分离株结合α-2,6唾液酸受体的能力较强，而H3和H6亚型LPAIV分离株主要表现为结合α-2,3唾液酸受体。本研究表明，鄱阳湖地区都昌县活禽市场中H3、H6和H9亚型LPAIV持续流行且存在混合感染，其中H9亚型LPAIV具有潜在的跨物种传播风险，建议加强活禽市场的病毒监测和环境清洗消毒。本研究不仅为鄱阳湖地区禽流感防控策略的制定提供了参考依据，也丰富了我国活禽市场禽流感病毒的流行数据。更多还原

【Abstract】 To investigate the prevalence of low pathogenic avian influenza virus(LPAIV) in live poultry markets(LPMs) in the Poyang Lake region of Jiangxi Province and to assess its public health risk, this study conducted continuous surveillance in the LPMs of Duchang County from June 2023 to May 2024. A total of 811 samples were collected from chickens, ducks, and environmental sources. Virus isolation was performed in chicken embryos, and pathogen detection was carried out to analyze the prevalence of LPAIV, the distribution of subtypes across different months, the source-specific distribution of LPAIV isolates, and the receptorbinding characteristics of different subtypes. The overall detection rate of LPAIV was 53. 4%(433/811), with the H9 subtype being the most prevalent(27. 5%, 223/811), followed by H6(9. 4%, 76/811) and H3(1. 6%, 13/811). Mixed infections involving multiple LPAIV subtypes were commonly observed, and H6 and H9 subtype LPAIV were detected every month. Environmental samples had the highest LPAIV detection rate(68. 9%), followed by chicken-derived samples(60. 2%) and duck-derived samples(41. 7%). H9 was the dominant subtype in chickens, whereas duck and environmental samples showed greater subtype diversity, mainly H3 and H6, along with mixed infections. Receptor-binding analysis revealed that H9 isolates showed stronger affinity for α-2,6 sialic acid receptors, indicating potential for cross-species transmission, while H3 and H6 isolates preferentially bound to α-2,3 sialic acid receptors. This study demonstrates that H3, H6, and H9 subtypes of LPAIV are persistently circulating in Duchang County’s live poultry markets, with frequent mixed infections. The H9 subtype, in particular, poses a potential risk for interspecies transmission. Strengthened surveillance and environmental sanitation in LPMs are recommended. The findings provide valuable reference data for avian influenza prevention strategies in the Poyang Lake region and enrich the national epidemiological database on avian influenza in live poultry markets.更多还原