【摘要】 为了探究柔嫩艾美耳球虫（Eimeria tenella）感染后不同球虫抗性鸡群体对免疫、氧化应激反应和炎症相关基因表达量的影响，本试验将京海黄鸡随机分为对照组和攻毒组，根据柔嫩艾美耳球虫感染后的相对增重率、粪便卵囊数、卵囊值、盲肠病变值和抗球虫指数等抗性评价指标，筛选出抗性组和易感组鸡群体，测定柔嫩艾美耳球虫感染后不同球虫抗性鸡群体血液炎性因子和抗氧化指标以及不同组织中炎性相关基因的表达量。结果显示，抗性组和易感组血液中超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性及活性氧（ROS）浓度与对照组相比差异极显著（P <0.01），但不同球虫抗性组间血液中SOD和CAT活性及ROS和一氧化氮（NO）浓度无显著差异（P> 0.05）；抗性组和易感组血液中白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、白细胞介素-1β(IL-1β)和肿瘤坏死因子α(TNF-α)浓度极显著高于对照组（P <0.01），且易感组IL-1β和TNF-α浓度极显著高于抗性组（P <0.01）；抗性组和易感组盲肠和胸腺中IL-6、IL-8、IL-1β和TNF-α基因表达量极显著高于对照组（P <0.01），且易感组盲肠和胸腺中IL-1β、TNF-α基因表达量极显著高于抗性组（P <0.01）。抗性组与易感组之间进行比较发现，2个组血液中IL-1β和TNF-α浓度与盲肠和胸腺组织中IL-1β和TNF-α基因表达量的变化规律高度一致。结果表明，柔嫩艾美耳球虫感染促进了不同球虫抗性鸡群体免疫、氧化应激反应和炎症相关基因表达量。更多还原

【Abstract】 To investigate the effects of Eimeria tenella infection on immune response, oxidative stress, and the expression of inflammation-related genes in chicken populations with different levels of coccidial resistance, this study randomly divided Jinghai yellow chickens into Control group and Infected group. Based on relative weight gain rate, fecal oocyst count, oocyst index, cecal lesion score, and anticoccidial index, chickens were screened into Resistant group and Susceptible group. The levels of inflammatory cytokines and antioxidant indicators in blood, as well as the expression of inflammation-related genes in various tissues, were measured following E. tenella infection. The results showed that both Resistance and Susceptible groups exhibited extremely significant different activities of superoxide dismutase(SOD), catalase(CAT), and concentration of reactive oxygen species(ROS) in blood compared with the Control group(P < 0. 01), but no significant differences in activities of SOD, CAT, and concentrations of ROS, nitric oxide(NO) were observed between the Resistance and Susceptible groups(P > 0. 05). However, the concentrations of interleukin-6(IL-6), interleukin-8(IL-8), interleukin-1β(IL-1β), and tumor necrosis factor-α(TNF-α) in the blood were extremely significant higher in both the Resistance and Susceptible groups than in the Control group(P < 0. 01).Furthermore, IL-1β and TNF-α levels were extremely significant higher in the Susceptible group than in the Resistance group(P <0. 01). Gene expression analyses in the cecal and thymus tissues showed that IL-6, IL-8, IL-1β, and TNF-α expression levels were extremely significant upregulated in both Resistance and Susceptible groups compared to the Control group(P < 0. 01), with IL-1βand TNF-α expression being extremely significant higher in the Susceptible group than in the Resistance group(P < 0. 01).Notably, the changes in IL-1β and TNF-α levels in blood corresponded closely with their expression trends in cecal and thymus tissues between the two groups. In conclusion, E. tenella infection significantly enhances immune activation, oxidative stress, and the expression of inflammation-related genes in chickens, with more pronounced effects in susceptible birds.更多还原