【摘要】 为了对4例疑似犬肿瘤的临床病例进行确诊，本文对临床送检的4例疑似犬肿瘤的肿物样本进行组织切片制作，经苏木精-伊红（H. E.）染色后，镜下可见增生的细胞排列呈束状、片状、巢状和螺纹状等，细胞呈梭形或多角形，部分细胞胞浆内含有黑色素颗粒，符合黑色素细胞肿瘤的特征；为了进一步观察细胞形态并对肿物进行确诊，使用黑色素瘤相关抗原（PNL-2）对肿瘤细胞进行免疫组织化学（IHC）染色，并用二氨基联苯胺（DAB）和3-氨基-9-乙基卡唑（AEC）显色液对其显色，结果均为恶性黑色素瘤（MM）。本试验通过对肿物的细胞形态学特征和IHC染色结果综合分析，将4例病例诊断为MM。试验结果不仅为犬MM的病理诊断提供参考，还为比较医学中MM的确诊提供参考依据。更多还原

【Abstract】 In order to accurately diagnose 4 suspected canine tumor clinical cases, this study examined tissue samples from 4suspected canine tumors sent for clinical testing. After hematoxylin-eosin(H. E.) staining, the microscopic examination revealed that the proliferating cells were arranged in bundles, sheets, nests, and spiral patterns, with cells being spindle-shaped or polygonal. Some cells contained melanin granules in their cytoplasm, which is characteristic of melanocytic tumors. To further investigate the cell morphology and confirm the diagnosis, melanoma-associated antigen(PNL-2) immunohistochemical(IHC) staining was performed on the tumor cells, followed by visualization using diaminobenzidine(DAB) and 3-amino-9-ethylcarbazole(AEC) chromogenic solutions. The results were consistent with malignant melanoma(MM). This study utilized a comprehensive analysis of the cell morphology and IHC staining results, diagnosing the 4 cases as MM. The findings not only provide a reference for the pathological diagnosis of canine MM but also offer valuable information for the diagnosis of MM in comparative medicine..更多还原