【摘要】 30只6～8周龄的雄性SPF级昆明小鼠被随机分为5组：空白对照组、模型组、地龙发酵物(Dilong)低剂量组、Dilong中剂量组、Dilong高剂量组；除空白对照组，其他小鼠连续3 d腹腔注射80 mg/kg环磷酰胺(cyclophosphamide, CTX)制备免疫抑制模型。造模结束后，空白对照组和模型组小鼠灌胃等量生理盐水，Dilong低、中、高剂量组分别灌胃100、200、400 mg/kg Dilong发酵物，治疗期为14 d,末次给药后2 h取其胸腺、脾脏、血液和肠道内容物。结果显示：CTX造成了试验期间小鼠体质量的下降，降低了胸腺指数以及血液中白细胞、淋巴细胞、粒细胞、免疫球蛋白水平，并造成了肠道菌群的紊乱；Dilong发酵物可有效缓解小鼠体质量和胸腺指数的降低，增加血液中免疫细胞和免疫球蛋白水平，改善肠道菌群多样性和丰富度，并增加有益菌丰度。结果表明，Dilong可有效缓解CTX造成的小鼠免疫抑制和肠道菌群紊乱，且以200 mg/kg添加量为最佳。更多还原

【Abstract】 Thirty SPF Kunming male mice aged 6—8 weeks were randomly divided into five groups:the control group,the model group,and the low,medium,and high dose groups of Dilong.Except for the control group,the other mice were continuously injected intraperitoneally with 80 mg/kg cyclophosphamide for 3 days to prepare an immunosuppressive model.After the modeling was completed,the control group and the model group were given an equal amount of physiological saline by gavage,while the low,medium,and high dose Dilong groups were given 100,200,and 400 mg/kg of Dilong fermented material by gavage,respectively.The treatment lasted for 14days,and thymus,spleen,blood,and intestinal contents were collected 2 hours after the last administration.The results showed that cyclophosphamide caused a weight loss in mice during the experiment,reduced thymus index,white blood cells,lymphocytes,granulocytes,immunoglobulin levels in the blood,and disrupted gut microbiota.Dilong fermented material can effectively improve the decrease in body weight and thymus index of mice,increase the levels of immune cells and immunoglobulin in the blood,improve the diversity and richness of intestinal microbiota,and increase the abundance of beneficial bacteria.Dilong fermented can effectively alleviate the immune suppression and intestinal microbiota disorder caused by cyclophosphamide in mice.Considering all factors,the optimal dosage is 200 mg/kg.更多还原