【摘要】 采用形态学筛选和聚合酶链式反应(PCR)从2010-2024年浙江省猪场送检的鼻拭子和肺脏组织样品中分离鉴定出119个多杀性巴氏杆菌临床分离株。用PCR技术和纸片琼脂扩散法(K-B)对分离的多杀性巴氏杆菌进行荚膜血清型分型、脂多糖血清型分型、毒力因子检测及耐药性分析。结果显示，荚膜血清型中A型有64株(占53.7%)、D型有54株(占45.3%)、F型有1株(占0.9%);脂多糖血清型中L1型有10株(占8.4%)、L3型有20株(占16.8%)、L6型有86株(占72.2%)、未定型有3株(占2.6%)。对10种毒力基因的扩增结果显示，毒力基因hgbA、hgbB和fimA的检出率在86.0%以上，toxA的检出率为8.4%,而毒力基因tbpA并未检出；不同毒力基因在不同荚膜血清型的分布情况和检出率也存在差异，pfhA只在荚膜血清A和F型中检出，在D型中并未发现；黏附素tadD在A型的检出率(92.2%)明显高于D型的检出率(9.3%),相反，黏附素hsf-1在D型的检出率(90.7%)明显高于A型的检出率(20.3%)。耐药性分析发现，多杀性巴氏杆菌对阿莫西林、氨苄西林、头孢类、强力霉素、氟苯尼考和环丙沙星等抗菌药物表现出较高的敏感性；对林可霉素、复方新诺明、庆大霉素及阿米卡星等抗菌药物的耐药性较强，多重耐药菌株有54株(78.3%)。综上，荚膜血清型以A和D型为主，脂多糖血清型以L6型为主，部分毒力基因在不同血清型间的分布差异较大，多重耐药菌株比例较高，为本病的防控提供了参考。更多还原

【Abstract】 The aim of this paper was to investigate the serotypes,virulence factors and drug resistance of clinical isolates of Pasteurella multocida of porcine origin in recent years.Morphological screening and polymerase chain reaction(PCR) were used to isolate and identify 119 isolates from nasal swabs and lung tissue samples sent from swine farms in Zhejiang Province from 2010 to2024.The isolates of Pasteurella multocida were subjected to capsular polysaccharide serotyping,lipopolysaccharide serotyping,virulence factor detection and drug resistance analysis by PCR and Kirby-Bauer disc agar diffusion method(K-B).The results showed that there were 64 strains(53.7%) of A-type,54 strains(45.3%) of D-type and 1 strain(0.9%) of F-type among the capsular polysaccharide serotypes,and 10 strains(8.4%) of Ll-type,20 strains(16.8%) of L3-type,86strains(72.2%) of L6-type,and 3 strains(2.6%) of undetermined type among the lipopolysaccharide serotypes.The amplification results of 10 virulence genes showed that the detection rate of virulence genes hgbA,hgbB and fimA was over 86.0%,the detection rate of toxA was 8.4%,while the virulence gene tbpA was not detected.There were also differences in the distribution virulence genes in different capsular polysaccharide serotypes.Virulence factor pfhA was detected in type A and F but not in type D.The detection rate of adhesin gene tadD in serotype A(92.2%)was significantly higher than that of type D(9.3%),and,on the contrary,the detection rate of adhesin gene hsf-1 in serotype D(90.7%) was significantly higher than that of type A(20.3%).Drug resistance analysis revealed that Pasteurella multocida showed high susceptibility to antimicrobial drugs such as amoxicillin,ampicillin,cephalosporins,doxycycline,fosfenicol and ciprofloxacin,and showed strong resistance to antimicrobial drugs such as lincomycin,cotrimoxazole,gentamicin and amikacin,and there were 54 multi-drug resistant strains(78.3%).In summary,capsular polysaccharide serotypes were dominated by type A and D,lipopolysaccharide serotypes were dominated by L6,the distribution of some virulence genes varied greatly among different serotypes,and the proportion of multi-resistant strains was high,which provide reference for the prevention and control of this disease.更多还原