【摘要】 探讨不同剂量橙皮苷(hesperidin, HP)对高脂饲喂联合无水乙醇动脉损伤诱导的兔动脉粥样硬化(atherosclerosis, AS)的作用及机制。选取6月龄健康雄兔25只，适应性饲喂1周，随机分为5组：对照组、模型组以及HP低、中、高剂量组。试验期间，对照组饲喂普通饲料；模型组饲喂高脂饲料30 d后颈总动脉灌注无水乙醇建立AS病理模型，继续高脂饲喂；HP低、中、高剂量组在模型组的基础上，在日粮中分别添加HP100、150和200 mg/kg,连续饲喂30 d。血细胞分析仪测定血细胞数目：白细胞(WBC)、淋巴细胞(lym)、单核细胞(Mon)、红细胞(RBC)、血红蛋白浓度(HGB)、血小板数目(PLT)和粒细胞(Gran);生化分析仪检测血脂4项水平：血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C);ELISA试剂盒检测氧化因子水平、炎性因子表达量。试验结束后，实验兔取颈动脉做病理组织切片HE、油红O和Masson染色，观察管壁结构与脂质浸润等情况。结果显示，模型与HP剂量组体质量显著上升(P<0.01);与模型组相比，HP干预显著降低了血液WBC、Mon、PLT的含量和Gran的占比(P<0.01);HP干预显著降低了兔血清炎性细胞因子TG、TC、LDL-C,并提高HDL-C水平(P<0.01);HP干预后血浆中TNF-α、IL-1含量极显著下降(P<0.01),IL-10水平显著上升(P<0.01);HP干预后SOD活性显著升高(P<0.01),MDA含量显著下降(P<0.01);组织切片可见HP干预能明显减少管壁脂质沉积、炎性细胞浸润，显著降低弹性纤维受损程度。结果表明：HP可通过调节血脂、炎症因子和氧化应激水平以减轻兔动脉粥样硬化。更多还原

【Abstract】 To explore the effects and processes of various hesperidin(HP) dosages on atherosclerosis(AS) in rabbits caused by high-fat diet in conjunction with anhydrous ethanol arterial damage.A total of twenty-five healthy male rabbits, six months of age, were chosen, given a week to acclimate and feed, and then randomly assigned to five groups: the model group, the control group, and the groups receiving low, middle, and high doses of HP.The control group received normal feed during the experiment; the model group received high-fat feed for 30 days, after which the common carotid artery was perfused with anhydrous ethanol to create a pathological model of AS;the HP low, middle, and high dose groups received high-fat feed continuously for 30 days based on the model group, with 100,150,and 200 mg/kg of HP added to the diets, respectively.The blood cell counts, including white blood cells(WBC),lymphocytes(lym),monocytes(Mon),red blood cells(RBC),hemoglobin concentration(HGB),platelet count(PLT),and granulocytes were measured using hematology analyzer.The serum total cholesterol(TC),triglycerides(TG),low density lipoprotein cholesterol(LDL-C),and high-density lipoprotein cholesterol(HDL-C) were analyzed using biochemical analyzer.An ELISA kit was used to measure the expression of inflammatory factors and the level of oxidative factors,while a blood cell analyzer was used to measure the quantity of blood cells and a biochemical analyzer was used to measure the level of four kinds of blood lipids.At the end of the test,the test rabbits were sacrificed to take carotid arteries for pathological tissue sections HE,Oil Red O and Masson staining to observe the structure of the wall with lipid infiltration and other conditions.The results indicated a significant increase in body weight in the model group compared to the HP dose group(P<0.01).In comparison to the model group,HP intervention significantly reduced the levels of WBC,Mon,PLT,and the percentage of Gran(P<0.01).Additionally,HP intervention significantly decreased serum inflammatory cytokines,including TG,TC,and LDL-C,while increasing HDL-C levels(P<0.01).The plasma levels of TNF-αand IL-1 decreased significantly following HP intervention(P<0.01),whereas the level of IL-10 increased significantly(P<0.01).Furthermore,SOD activity was significantly higher(P<0.01)and MDA content was significantly lower(P<0.01)after HP intervention.Histological examination revealed that HP intervention significantly reduced lipid deposition in tubular walls,inflammatory cell infiltration,and the extent of elastic fiber damage.These results suggest that HP may attenuate atherosclerosis in rabbits by modulating lipid levels,inflammatory factors,and oxidative stress.更多还原