【摘要】 为探讨DPP4-Nestin轴在泡球蚴感染引起肝纤维化过程中的作用，选取C57BL/6小鼠通过肝门静脉构建泡球蚴感染模型，HE法检测肝脏组织病理学变化，免疫组化、免疫荧光检测Nestin及DPP4在泡球蚴感染小鼠肝脏中的表达；以DPP4重组蛋白刺激小鼠肝星状细胞系JS1构建体外模型，qPCR、Western blot、shRNA慢病毒干扰等方法检测DPP4-Nestin轴在肝星状细胞激活过程中的作用。结果显示：与Sham组相比，泡球蚴感染组肝脏组织结构破坏，胶原沉积明显，Nestin和DPP4表达显著升高(P<0.050 0),且Nesin与α-SMA存在共定位；与对照组相比，DPP4重组蛋白刺激可明显刺激JS1细胞激活(P<0.050 0),并且Nestin表达升高(P<0.050 0),shRNA慢病毒抑制JS1细胞Nestin表达可明显抑制DPP4重组蛋白对JS1细胞的活化作用(P<0.050 0)。综上所述，DPP4-Nestin轴在泡球蚴感染引起肝星状细胞活化过程中具有重要调节作用，靶向DPP4-Nestin轴可作为治疗泡球蚴感染所致肝纤维化的新途径。更多还原

【Abstract】 To investigate the role of the DPP4-nestin axis in liver fibrosis induced by alveolar cyst infection, a murine model was established using C57BL/6 mice via hepatic portal vein injection.Liver histopathological changes were assessed using HE staining, while immunohistochemistry and immunofluorescence were employed to evaluate the expression levels of nestin and DPP4 in infected mouse livers.In vitro,JS1 cell line was stimulated with recombinant DPP4 protein to establish a cellular model,and qPCR,Western blot,and shRNA lentivirus interference techniques were utilized to examine the involvement of the DPP4-nestin axis in hepatic stellate cell activation.The findings demonstrated that compared to the Sham group,liver tissue structure disruption and collagen deposition were evident along with significantly increased expressions of nestin and DPP4(P<0.050 0),which colocalized with nesin andα-SMA.Furthermore,stimulation with recombinant DPP4 protein significantly enhanced JS1 cell activation(P<0.050 0)as well as upregulated nestin expression(P<0.050 0)when compared to control group cells.Notably,shRNA lentivirusmediated inhibition of nestin expression effectively suppressed the activating effects exerted by recombinant DPP4 protein on JS1 cells(P<0.050 0).Collectively,these results highlight the crucial regulatory role played by the DPP4-nestin axis in hepatic stellate cell activation triggered by alveolar infection;thus,targeting this axis may represent a novel therapeutic strategy for treating alveolar infection-induced liver fibrosis.更多还原