【摘要】 试验以收集的11个紫芝为材料，从菌落特征、拮抗试验、分子标记等方面进行紫芝遗传差异评价。结果表明，11个菌株在菌落形态特征、裂解培养基的能力、菌丝分泌物等方面表现出多样性。拮抗试验结果显示，菌株Gs0010与Gs0012、Gs0009与Gs0014间无拮抗反应；其余菌株间均发生不同程度的拮抗反应。在发生拮抗反应的菌株间，拮抗线呈现隔离型、隔离型带线状和墙式结构3种类型。通过RAPD、ISSR和SRAP这3种分子标记技术对11个菌株的遗传差异进行分析，结果显示菌株Gs0010与Gs0012、Gs0009与Gs0014的遗传相似系数为1.00,且各在系统发育树中聚为一支，结合菌落特征和拮抗试验结果，可判断这2组菌株分别为同一菌株。除此之外的各菌株间均存在遗传差异，且差异大小与菌株地理来源有关。更多还原

【Abstract】 11 collected samples of Ganoderma sinense were used as materials to evaluate the genetic differences through colony characteristics, antagonistic tests, and molecular markers. The results indicated that the 11 strains exhibited diversity in terms of colony morphological characteristics, the ability to lysate the medium, and mycelial secretions. The results of the antagonistic test showed that there was no antagonistic reaction between strains Gs0010 and Gs0012,Gs0009 and Gs0014. Antagonistic reactions of varying degrees occurred among the other strains. Among the strains that undergo antagonistic reactions, the antagonistic lines present three types: isolation type, band-like isolation structure, and wall-like structure. The genetic differences of 11 strains were analyzed by three molecular marker techniques, namely RAPD,ISSR and SRAP. The results showed that the genetic similarity coefficients of strains Gs0010 and Gs0012,Gs0009 and Gs0014 were 1.00,and they clustered into one branch in the phylogenetic tree. Combined with the colony characteristics and the results of the antagonism test, it can be determined that these two groups of strains are the same strain respectively. In addition, there are genetic differences among the other strains, and the extent of these differences is related to the strains’ origin.更多还原