【摘要】 目的 旨在分析重庆市鲜活淡水鱼虾中丁香酚残留水平，并评估其对人群造成的健康风险。方法 采用同位素内标-超高效液相色谱-三重四极杆质谱联用法测定121份鲜活淡水鱼虾中丁香酚的含量，分析其残留水平。结合我市不同人群的鱼虾消费数据，通过设定暴露情景，估算出丁香酚的每日估计摄入量（EDI）。并采用每日允许摄入量（ADI）标准进行风险评估，参考标准分别为JECFA（2006）提出的2.5 mg/（kg·BW/d）和EFSA（2012）设定的1.0 mg/（kg·BW/d）。结果 121份鲜活鱼虾样品中仅淡水鱼中检出了丁香酚残留，96份淡水鱼中丁香酚检出率68.75%，残留水平为n. d～11.9 mg/kg,25份淡水虾均未检出丁香酚；从某农贸市场采集的草鱼样本中检测出最高丁香酚含量，达11.9 mg/kg。在鱼类中，草鱼、鲢鱼和鲫鱼的丁香酚检出率分别为78.12%、78.26%和58.33%，平均含量依次为0.637、0.363和0.197 mg/kg。统计分析表明，农贸市场鱼样的丁香酚平均浓度（0.557 mg/kg）与检出率（76.00%）均显著高于零售店样本（0.236 mg/kg,60.87%）（P<0.05）。3岁以上人群经鱼类膳食摄入丁香酚的EDI为0.16×10^-3～8.82×10^-3 mg/（kg·BW/d），占ADI的比例不足1%。结论 重庆市鲜活淡水鱼中存在丁香酚残留，鲜活淡水虾尚未检出丁香酚。基于当前监测水平，经淡水鱼肉摄入丁香酚的健康风险较低。更多还原

【Abstract】 Objective The aim of this study was to analyze the concentrations of eugenol residues in live freshwater fish and shrimp, and to evaluate potential human health risks associated with their consumption in Chongqing City.Methods The eugenol concentrations of 121 fresh freshwater fish and shrimp samples were determined using ultra-performance liquid chromatography-triple quadrupole mass spectrometry（UPLC-MS/MS） with isotope-labeled internal standard. The estimated daily intake（EDI） of eugenol from consuming these products was calculated based on food consumption data. Risk assessments were performed using the acceptable daily intake（ADI） values of 2. 5 mg/（kg·BW/d）（JECFA, 2006） and 1. 0 mg/（kg·BW/d）（EFSA, 2012）.Results The detection rate of eugenol in fish samples was 68. 75%（66/96）, with an average level of 0. 403 mg/kg. No eugenol was detected in all 25 shrimp samples. The highest eugenol level（11. 9 mg/kg） was found in a grass carp sampled from an agricultural product market. For fish species, the detection rates of eugenol in grass carp, hypophthalmichthys molitrix, and crucian carp were 78. 12%, 78. 26%, and 58. 33% respectively, with average concentrations of 0. 637, 0. 363, and 0. 197 mg/kg. Fish samples from agricultural product markets had significantly higher eugenol concentration（0. 557 mg/kg） and detection rate（76. 00 %） than those from retail stores（0. 236 mg/kg, 60. 87%）（P<0. 05）. The EDI of eugenol through fish diet for individuals aged above 3 years old is between 0. 16×10^-3～8. 82×10^-3 mg/（kg·BW/d）, accounting for less than 1% of the ADI.Conclusion Eugenol residues were detected in live freshwater fish samples other than shrimp samples in Chongqing City. Based on current monitoring data, the health risks associated with eugenol in live freshwater fish are relatively low.更多还原