【摘要】 为提高连翘叶中连翘苷和连翘酯苷A的提取率，以连翘叶为实验材料用超声法、微波法、浸提法、回流法4种方法提取连翘叶中连翘苷和连翘酯苷A，采用高效液相法测定连翘苷和连翘酯苷A的含量。以连翘苷和连翘酯苷A含量之和的均值为综合评价指标，筛选适宜的提取方法。以液料比、乙醇浓度、超声时间为考察因素，利用正交实验与极差分析进一步优化提取工艺。结果表明，超声法所得的提取液中连翘苷和连翘酯苷A的综合评分指标最高，超声法适宜作为连翘叶中连翘苷和连翘酯苷A的提取方法，优化后的提取工艺中乙醇浓度为60%，超声时间为25 min，料液比为1∶20。更多还原

【Abstract】 The aim of this study is to optimize the extraction process of phillyrin and forsythiaside A from Forsythia suspensa leaves and enhance their extraction yields,this study adopted four extraction methods,namely ultrasonic method,microwave method,extraction method and reflux method,to extract phillyrin and forsythiaside A from the leaves. High-performance liquid chromatography was used to determine the content of phillyrin and forsythiaside A. The average value of the sum of forsythiaside A and phillyrin was used as the comprehensive evaluation index to screen the appropriate extraction method and to further optimize it.Taking the liquid-to-material ratio,ethanol concentration and ultrasonic time as the investigation factors,the extraction process was optimized by orthogonal experiments and range analysis. The results showed that the comprehensive evaluation score was the highest when utilizing the ultrasonic method,indicating that the ultrasonic method is the best extraction method,with the optimized process of ethanol concentration of 60%,ultrasonic time of 25 minutes,and solid-liquid ratio of 1∶20.更多还原