【摘要】 目的 分析牛黄解毒软胶囊的化学成分及入血成分，阐明其可能的药效物质基础。方法 采用超高效液相色谱-高分辨质谱联用(UPLC-HRMS)技术和Xcalibur 4.0质谱工作站中的Qual Browser软件，对正、负离子模式下的质谱信息进行数据采集和处理，结合保留时间、特征碎片、分子式等信息，通过文献、OTCML数据库以及对照品信息进行综合比对，鉴定牛黄解毒软胶囊的体内外成分。结果 从牛黄解毒软胶囊中共鉴定出110个化学成分，在大鼠血浆样品中检测到了其中的66个化学成分。这些成分主要包括黄酮类、三萜类、胆汁酸类、蒽醌类、皂苷、有机酸类、多酚类、甾体、二苯乙烯苷等，其中黄酮类化合物数量最多。结论 本研究首次应用UPLC-HRMS技术对牛黄解毒软胶囊中的主要化学成分及原形入血成分进行分析，为揭示牛黄解毒软胶囊的药效物质基础提供了重要信息，同时也为该药品的进一步质量控制和深入研究奠定了基础。更多还原

【Abstract】 Objective To determine the chemical components and prototype blood components of Niuhuang Jiedu soft capsules and elucidate their potential pharmacodynamic material basis.Methods Ultra-performance liquid chromatography coupled with high-resolution mass spectrometry (UPLC-HRMS) was used,along with the Qual Browser software in the Xcalibur 4.0 mass spectrometry workstation,to acquire and process mass spectrometry data in both positive and negative ion modes.By integrating the retention time,characteristic fragments,molecular formulas,and other information,the components of Niuhuang Jiedu soft capsules were identified through comprehensive comparison with literature,the OTCML database,and reference standards.Results Totally 110 chemical components were identified in Niuhuang Jiedu soft capsules,66 of which were detected in rat plasma samples.These components primarily included flavonoids,triterpenoids,bile acids,anthraquinones,saponins,organic acids,polyphenols,sterols,and stilbene glycosides,with flavonoids being the most abundant.Conclusion This study first applies UPLC-HRMS technology to analyze the main chemical components and prototype blood components in Niuhuang Jiedu soft capsules.The findings provide crucial insights into the pharmacodynamic material basis of the capsules and lay a foundation for further quality control and in-depth research on this product.更多还原