【摘要】 目的 探究甘草地上部分黄酮类成分抗慢性前列腺炎的有效成分及可能作用机制。方法 采用超高效液相图谱-四极杆-静电场轨道阱高分辨质谱(UPLC-Q-Exactive Orbitrap-MS)鉴定甘草地上部分醇提物中的主要黄酮类化学成分，利用网络药理学和分子对接，预测甘草地上部分抗慢性前列腺炎的核心成分与关键靶点。采用角叉菜胶诱导构建慢性前列腺炎大鼠模型，通过HE染色观察大鼠前列腺组织病理变化，ELISA法检测血清中双氢睾酮(DHT)、干扰素-γ(IFN-γ)、免疫球蛋白G(IgG)、白细胞介素-6(IL-6)、前列腺特异抗原(PSA)、肿瘤坏死因子-α(TNF-α)水平，评价甘草地上部分醇提物对慢性前列腺炎的治疗效果。结果 从甘草地上部分醇提物中共鉴定出44种黄酮类化合物，通过网络药理学分析，预测甘草素、毛蕊异黄酮、刺芒柄花素、甘草苷等为核心成分，通过作用于AKT1、TNF、VEGFA、EGFR、MMP9等关键靶点，调控相关信号通路，发挥抗慢性前列腺炎作用。分子对接验证显示核心成分与关键靶点具有较好的结合能力。动物实验结果表明甘草地上部分醇提物对大鼠慢性前列腺炎有较好的治疗作用。结论 本研究验证了甘草地上部分醇提物的抗慢性前列腺炎药效，其活性成分为刺芒柄花素、毛蕊异黄酮、甘草素等，可能通过PI3K-AKT信号通路，调控EGFR、AKT1、MMP9等关键靶点发挥治疗功效。更多还原

【Abstract】 Objective To determine the active ingredients and possible mechanism of action of flavonoids from aerial parts of Glycyrrhiza uralensis for chronic prostatitis.Methods Ultra performance liquid chromatography-quadrupole-electrostatic field orbitrap high-resolution mass spectrometry (UPLC-Q-Exactive Orbitrap-MS) was used to identify the main flavonoid components in the alcoholic extract of aerial parts of Glycyrrhiza uralensis,and to predict corresponding core components and key targets for chronic prostatitis with network pharmacology and molecular docking.A rat model of chronic prostatitis was induced by carrageenan gum,and the histopathological changes of rat prostate were observed by HE staining.The levels of DHT,IFN-γ,IgG,IL-6,PSA,and TNF-α were detected in the serum by ELISA,to evaluate their therapeutic efficacy extract on chronic prostatitis.Results Totally 44 flavonoids were identified in the alcoholic extracts.Through the network pharmacological analysis,glycyrrhizin,trichothecene isoflavones,prickly manzanita florifera,and glycyrrhizin were predicted to be the core components,which exerted the antichronic prostatitis effects by acting on the key targets,such as AKT1,TNF,VEGFA,EGFR,and MMP9,and regulating the related signaling pathways.The molecular docking validation showed that the core components had better binding ability with the key targets.The animal experiments showed that the alcoholic extract of aerial parts of Glycyrrhiza uralensis had a fairly good therapeutic effect on chronic prostatitis in rats.Conclusion The anti-chronic prostatitis efficacy of aerial parts of Glycyrrhiza uralensis.is verified.The active components included spiny aristolochicin,trichostatin isoflavones,and glycyrrhizin,whose therapeutic efficacy may through the PI3K-AKT signaling pathway,regulate the key targets of EGFR,AKT1,and MMP9.更多还原