【摘要】 目的 通过体内外实验探讨薏苡附子败酱散对肝癌术后复发模型的影响及机制。方法 制备大鼠薏苡附子败酱散含药血清，采用MTT法检测细胞增殖活性，采用划痕实验和Transwell实验检测细胞迁移和侵袭能力。建立H22原位移植瘤术后复发小鼠模型，观察各组小鼠腹围变化、肝结节数量，测量瘤块总重量，检测最大瘤块体积；酶联免疫吸附法(ELISA)测定血清中甲胎蛋白(AFP)、碱性成纤维细胞生长因子(bFGF)和血管内皮生长因子(VEGF)水平；苏木精-伊红染色(HE)观察肝癌复发的病理情况；免疫荧光检测微血管增生情况及Wnt/β-catenin调节通路的变化。结果 薏苡附子败酱散含药血清可明显抑制HepG2细胞增殖，增加划痕实验愈合面积，减少HepG2细胞穿孔数量。薏苡附子败酱散可明显改善术后复发模型小鼠表观指标及肝脏组织病理变化；降低血清AFP、bFGF和VEGF水平；降低癌旁组织中VEGF、CD34和CD44v6的表达；抑制Wnt/β-catenin调节通路中GSK3β、Wnt1和β-catenin的表达。结论 薏苡附子败酱散具有抑制小鼠H22原位移植瘤肝癌术后复发的作用，其机制可能与抑制Wnt/β-catenin介导的微血管增生有关。更多还原

【Abstract】 Objective To determine the effect and mechanism of Yiyi Fuzi Baijiang powder on the postoperative recurrence of liver cancer by in vivo and in vitro experiments.Methods The Yiyi Fuzi Baijiang powder-containing serum was prepared.The cell proliferation activity was detected by MTT assay.The cell migration and invasion abilities were detected by wound healing assay and Transwell assay.A postoperative recurrence model of orthotopically transplanted H22 tumors in mice was established to observe the changes in abdominal circumference and the number of hepatic nodules.The total tumor weight and the maximum tumor volume were measured.The levels of alpha-fetoprotein (AFP),basic fibroblast growth factor (bFGF),and vascular endothelial growth factor (VEGF) in the serum were determined by enzyme-linked immunosorbent assay (ELISA).Hematoxylin and eosin (HE) staining was used to observe the pathological changes in the liver tissue.The microvascular proliferation and changes in the Wnt/β-catenin regulatory pathway were detected by immunofluorescence staining.Results The Yiyi Fuzi Baijiang powder-containing serum significantly inhibited the proliferation of HepG2 cells,increased the healing area in the wound healing assay,and reduced the number of perforations in HepG2 cells.Yiyi Fuzi Baijiang powder greatly improved apparent indicators and histological changes in the liver tissue of mice in a postoperative recurrence model,decreased the levels of AFP,bFGF,and VEGF in the serum,reduced the expression of VEGF,CD34,and CD44v6 in adjacent tissues,and inhibited the expression of GSK3β,Wnt1,and β-catenin in the Wnt/β-catenin regulatory pathway.Conclusion Yiyi Fuzi Baijiang powder can inhibit the postoperative recurrence of orthotopically transplanted H22 tumors in mice,its mechanism may be related to the inhibition of Wnt/β-catenin-mediated microvascular proliferation.更多还原