【摘要】 分离鉴定番茄种子内生细菌功能菌株，为开发高效微生物肥料奠定基础。本研究以番茄种子为试材，采用稀释涂布平板法进行内生细菌的分离纯化，通过16S rRNA基因序列分析确定菌株的系统发育分类，并利用CAS平板检测法、无机磷培养基定性检测法及Salkowski比色法测定菌株的促生特性，同时通过发芽试验分析吲哚乙酸(IAA)产生菌株对番茄种子萌发的影响。16S rRNA基因序列分析结果表明，从番茄种子中分离纯化获得的9株内生细菌，分属于肠杆菌属(Enterobacter)与芽孢杆菌属(Bacillus)。促生特性检测结果表明，4株菌具有产铁载体能力，3株菌可产生IAA，其中B2和B4菌株的IAA产量较高，分别为88.30μg/mL与69.31μg/mL，且两株菌均表现出溶解无机磷的特性。发芽试验进一步证实，B4菌株发酵液能够显著促进番茄种子萌发。本研究丰富了番茄种子内生细菌菌种资源，筛选出的B4菌株可作为潜在的促生菌资源用于多功能菌肥开发。更多还原

【Abstract】 Isolation and identification of functional endophytic bacterial strains from tomato seeds to establish a foundation for developing high-efficiency microbial fertilizers. Tomato seeds were used as the experimental material in this study. Endophytic bacteria were isolated and purified from the seeds using the dilution spread plate method. The phylogenetic analysis of strains was done by partial 16S rRNA gene sequences. The promotion characteristics of the strains were assessed via CAS plate assays, inorganic phosphate plate assays,and the Salkowski colorimetric method. Additionally, a seed germination assay was conducted to evaluate the effects of indole acetic acid（IAA）-producing bacterial strains on tomato seed germination. The results of 16S rRNA gene sequence analysis revealed that nine isolated endophytic bacterial strains from tomato seeds belong to two genera: Enterobacter and Bacillus. The plant growth-promoting trait assays revealed that four strains possessed siderophore-producing ability, and three strains exhibited IAA production ability, among which strains B₂ and B₄ demonstrated higher IAA yields of 88.30 μg/mL and 69.31 μg/mL, respectively. Notably,both strains also exhibited the ability to dissolve inorganic phosphorus. Germination assays further confirmed that the fermented broth of strain B₄ significantly promoted tomato seed germination. This study enriches the tomato endophytic bacterial resource library, and the screened B₄ strain demonstrates potential as a plant growth-promoting resource for developing multi-functional microbial fertilizers.更多还原