【摘要】 为了探明外源物质对茄腐镰刀菌(Fusarium solani)引发的三七根腐病的协同防治效果，本研究采用L-赖氨酸和哈茨木霉菌(Trichoderma harzianum)配合施用，开展室内带药平板和室外盆栽试验，明确不同处理对F. solani菌株、三七幼苗生长、根腐病防控及其对三七根际微生物多样性调控的影响。结果表明：1μmol/L L-赖氨酸对F. solani的抑制率仅有13.32%，但显著促进了T. harzianum生长(23.05%)和产孢(173.61%)；盆栽试验中，以LTF1处理(1μmol/L L-Lysine+T. harzianum+F. solani)应用效果最好；与CK(F. solani)相比，三七存苗率、根鲜重和根干重分别提高45.83%、27.45%和33.33%，根腐病发病率降低88.89%。根际微生物高通量测序分析表明，对土壤真菌和细菌多样性均有影响，其中，真菌群落丰度降低最为显著。子囊菌门(Ascomycota,87.27%)和放线菌门(Actinobacteriota,24.54%)为优势菌群，木霉属(Trichoderma,28.57%)、厌氧粘细菌属(Anaeromyxobacter,2.47%)及类芽孢杆菌属(Paenibacillus,1.96%)等功能菌属显著富集，与根腐病发病率呈负相关。本实验结果为L-赖氨酸和哈茨木霉菌(Trichoderma harzianum)联用防控三七根腐病提供生态治理新策略。更多还原

【Abstract】 To investigate the synergistic control effect of exogenous substances on Panax notoginseng root rot disease caused by Fusarium solani, this study conducted in vitro plate culture method and outdoor pot experiments using combined application of L-Lysine and Trichoderma harzianum, aiming to clarify the effects of different treatments on F. solani strain, P. notoginseng seedling growth, root rot disease control, and their regulation on rhizosphere microbial diversity. The results showed that: 1 μmol/L L-Lysine exhibited only13.32% inhibition rate against F. solani, but significantly promoted the mycelial growth(23.05%) and spore production(173.61%) of T. harzianum. In pot experiments, the LTF1 treatment(1 μmol/L L-Lysine + T.harzianum+F. solani) demonstrated the optimal application effect. Compared with CK(F. solani), the survival rate, root fresh weight and root dry weight of P. notoginseng increased by 45.83%, 27.45% and 33.33%respectively, while the root rot incidence decreased by 88.89%. High-throughput sequencing analysis of rhizosphere microbes revealed impacts on both fungal and bacterial diversity, with the most significant reduction observed in fungal community abundance. Ascomycota(87.27%) and Actinobacteriota(24.54%)emerged as dominant phyla, while functional genera including Trichoderma(28.57%), Anaeromyxobacter(2.47%) and Paenibacillus(1.96%) showed significant enrichment, exhibiting negative correlations with root rot incidence. This study provides new ecological management strategies for controlling P. notoginseng root rot disease through combined application of L-Lysine and Trichoderma harzianum.更多还原