【摘要】 探究β-葡聚糖酶对辣椒植株防御酶活性的影响，及对辣椒常见土传病害的生物防治作用，为辣椒土传病害生物防治提供参考。采用琼脂糖凝胶色谱柱层析法纯化所得的β-葡聚糖酶液对辣椒进行浇灌处理，分别于辣椒幼苗期、初花期、坐果期测定辣椒幼苗叶片组织中多种防御酶的活性以及丙二醛的含量；采用人工接种病原菌的方法，分别于苗期和坐果期考察不同浓度β-葡聚糖酶处理对辣椒疫病、辣椒枯萎病和辣椒炭疽病的生防效果。结果表明，β-葡聚糖酶对谷胱甘肽还原酶活性没有影响，但对辣椒超氧化物歧化酶、过氧化物酶、多酚氧化酶、苯丙氨酸解氨酶、过氧化氢酶和抗坏血酸过氧化物酶的活性均有不同程度的促进作用，同时对丙二醛含量有明显的抑制作用；β-葡聚糖酶液稀释10～100倍处理对辣椒疫病、辣椒炭疽病和辣椒枯萎病的防治效果分别达47.08%～82.23%、42.35%～70.25%和44.21%～65.92%。综上，β-葡聚糖酶能够提高辣椒防御酶的活性，增强植株的抗性，对辣椒疫病、辣椒炭疽病和辣椒枯萎病具有明显的生物防治效果。此外，β-葡聚糖酶作为一种生物防治制剂，不会使病原菌产生抗药性，且对环境友好，具有开发成新型生物农药用于辣椒土传病害防治的前景。更多还原

【Abstract】 The study aims to explore the effect of β-glucanase on the activity of defense enzymes in pepper plants and its biological control effect on common pepper soil borne diseases, providing new reference directions for the biological control of pepper soil borne diseases. Pepper seedlings were treated with β-glucanase solution purified by agarose gel chromatography. The activities of SOD, POD, PPO, PAL, CAT, APX and GR in the leaves of pepper seedlings were measured at the seedling stage, the first flowering stage and the fruit setting stage, respectively, and the content of MDA was measured simultaneously. Artificial inoculation was used to investigate the biocontrol effects of different concentrations of β-glucanase treatment on pepper blight, pepper wilt, and pepper anthracnose during the seedling and fruit setting stages. The research results showed that β-glucanase treatment had no effect on GR activity, but promoted the activities of SOD, POD,PPO, PAL, CAT, and APX to varying degrees in peppers, and β-glucanase treatment also had a significant inhibitory effect on MDA content. The control effects of β-glucanase diluted 10-100 times on pepper blight,pepper anthracnose, and pepper wilt were 47.08%-82.23%, 42.35%-70.25%, and 44.21%-65.92%,respectively. β-glucanase can increase the activity of pepper defense enzymes, enhance plant resistance, and has significant biocontrol effects on pepper blight, pepper anthracnose, and pepper wilt. As a biocontrol agent,β-glucanase is environmentally friendly and does not cause insect resistance, making it a promising new biopesticide for controlling pepper soil borne diseases.更多还原