【摘要】 为减少苹果果渣（AP）浪费，该研究以林芝苹果渣为原料，采用酵母菌BV818、戊糖乳杆菌（Lactobacillus pentosus）顺序发酵制备苹果渣酵素（APJS）。以酵母菌、戊糖乳杆菌活菌数和超氧化物歧化酶（SOD）活性为评价指标，通过响应面试验和正交试验优化酵母菌、戊糖乳杆菌发酵条件，并分析苹果渣酵素的理化指标和抗氧化能力。结果表明，优化酵母菌发酵条件为白砂糖添加量5%、发酵温度25℃、酵母菌接种量0.20%。此条件下，酵母菌活菌数、SOD活性分别为8.79 lg(CFU/m L)、932.95 U/m L；优化戊糖乳杆菌发酵条件为戊糖乳杆菌接种量1.5%、发酵时间36 h，发酵温度37℃。此条件下，戊糖乳杆菌活菌数、SOD活性分别为7.67 lg(CFU/m L)、2 618.02 U/m L。APJS可溶性固形物、总糖、还原糖含量及pH值分别为7.23°Bx、16.25 mg/m L、0.74 mg/m L、3.61，与AP相比，均显著下降（P<0.05）;APJS总酸、总黄酮、γ-氨基丁酸（GABA）含量、1,1-二苯基-2-三硝基苯肼（DPPH）、2,2’-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）自由基清除率及铁离子还原/抗氧化能力（FRAP）值分别为48.19 mg/g、0.46 mg/m L、11.64μg/m L、60.57%、78.12%、1.36，与AP相比，均显著升高（P<0.05)。更多还原

【Abstract】 To reduce the waste of apple pomace(AP), using apple pomace from Linzhi as raw material, apple pomace Jiaosu(APJS) was prepared by sequential fermentation with BV818 yeast and Lactobacillus pentosus. Taking the viable yeast and Lactobacillus pentosus counts, and superoxide dismutase(SOD) activity as evaluation indicators, the fermentation conditions for yeast and L. pentosus were optimized by response surface tests and orthogonal tests, and the physicochemical indexes and antioxidant capacity of apple pomace Jiaosu were analyzed. The results showed that the optimal fermentation conditions for yeast were as follows: sugar addition 5%, fermentation temperature 25 ℃, and yeast inoculum 0.20%. Under these conditions, the viable yeast counts and SOD activity reached 8.79 lg(CFU/ml) and 932.95 U/ml, respectively. The optimal fermentation conditions for L. pentosus were as follows: L. pentosus inoculum 1.5%, fermentation time 36 h and temperature 37 ℃. Under these conditions, the viable counts of Lactobacillus pentosus and SOD activity were 7.67 lg(CFU/ml) and 2 618.02 U/ml, respectively. The soluble solids, total sugar, reducing sugar, and pH of APJS were 7.23 °Bx, 16.25 mg/ml, 0.74 mg/ml and 3.61, respectively, which were significantly decreased compared with AP(P<0.05). The total acid,total flavonoids, γ-aminobutyric acid(GABA), 1,1-diphenyl-2-picrylhydrazyl(DPPH), 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)(ABTS)radical scavenging rates, and ferric reducing/antioxidant power(FRAP) value of APJS were 48.19 mg/g, 0.46 mg/ml, 11.64 μg/ml, 60.57%, 78.12%and 1.36, respectively, all of which were significantly increased compared with AP(P<0.05).更多还原