【摘要】 该研究以一株筛选自西藏开菲尔粒中的乳酸菌W114为研究对象，采用形态学观察、生理生化实验及分子生物学技术对其进行鉴定，并研究其后生元的制备方法和抗氧化、酪氨酸酶抑制等特性，进一步观察其对维护皮肤微生态的影响。结果表明，菌株W114被鉴定为植物乳植杆菌（Lactiplantibacillus plantarum）；当后生元添加量为10%(V/V)时，对1,1-二苯基-2-三硝基苯肼（DPPH）自由基清除能力为36.42%，对酪氨酸酶的抑制率达到51.91%；随机抽取10名受试者使用含有菌株W114的后生元面膜，16S r DNA高通量测序结果表明，受试者的面部皮肤菌群丰富度均显著升高（P<0.05），菌群分布更为集中，能调节与炎症、衰老、皮肤屏障等相关的菌群丰度，使皮肤菌群中放线菌门（Actinobacteria）、厚壁菌门（Firmicutes）和变形菌门（Proteobacteria）总相对丰度增加，链球菌属（Streptococcus）相对丰度降低，表面葡萄球菌（Staphylococcus epidermidis）和人葡萄球菌（Staphylococcus hominis）的相对丰度升高，有效改善了皮肤的微生态。更多还原

【Abstract】 Using a lactic acid bacterium W114 selected from Kefir granules in Tibet as the research object, the strain was identified by morphological observation, physiological and biochemical experiments, and molecular biology techniques, the preparation methods of its postbiotics, antioxidant and tyrosinase inhibitory properties were studied, and its effect on maintaining the microecology of the skin was observed. The results showed that the strain W114 was identified as Lactiplantibacillus plantarum. When the postbiogens addition was 10%(V/V), the scavenging ability of 1,1-diphenyl-2-picrylhydrazyl(DPPH) free radicals was 36.42%, and the tyrosinase inhibitory rate reached 51.91%. Ten subjects were randomly selected to use postbiotics masks containing strain W114. The results of 16S r DNA high-throughput sequencing showed that the richness of facial skin flora in the subjects was significantly increased(P<0.05), and the distribution of flora was more concentrated, which could regulate the abundance of flora related to inflammation, aging, skin barrier, etc., increase the total relative abundance of Actinobacteria, Firmicutes and Proteobacteria in the skin flora, reduce the relative abundance of Streptococcus, and increase the relative abundance of Staphylococcus epidermidis and Staphylococcus hominis, effectively improve the microecology of the skin.更多还原