【摘要】 目的 探讨桑椹水提物促进骨髓间充质干细胞（BMSCs）成软骨细胞分化的机制。方法 全骨髓贴壁法提取BMSCs,CCK8法检测桑椹水提物对细胞增殖的影响，RT-qPCR筛选桑椹水提物促BMSCs软骨分化中miRNAs的变化，生物学信息预测及双荧光素酶报告基因验证rno-miR-139靶向调控MMP14,RT-qPCR及WB检测rno-miR-139 mimic和inhibitor对MMP14表达的影响。siMMP14转染BMSCs，检测Sox9的表达。免疫组化检测rno-miR-139对体内异位移植的BMSCs成软骨分化的影响。结果 桑椹水提物有效促BMSCs成软骨细胞分化中rno-miR-139靶向负调控MMP14，并促进Sox9表达，BMSCs体内异位软骨分化中rno-miR-139下调促进Sox9、CollagenⅡ表达。结论 桑椹水提物抑制rno-miR-139上调MMP14促BMSCs成软骨分化。更多还原

【Abstract】 Objective To investigate the mechanism of mulberry aqueous extract promoting chondrogenic differentiation of bone marrow mesenchymal stem cells(BMSC). Methods BMSCs were extracted by the whole bone marrow adherent method. The CCK8 assay was used to detect the proliferation of BMSCs induced by mulberry aqueous extract on cells. RT-qPCR was used to test miRNAs in BMSCs treated by mulberry aqueous extract. Biological information prediction and the dual luciferase reporter gene validated that MMP14 was regulated by rno-miR-139. RT-qPCR and WB were used to detect the effect of rno-miR-139mimic and inhibitor on MMP14 expression. siMMP14 transfected BMSCs to detect the expression of Sox9.Immunohistochemistry was used to detect rno-miR-139 regulate chondrogenic differentiation of BMSCs in ectopic transplantation in vivo. Results The mulberry aqueous extract effectively promoted the chondrogenic differentiation of BMSCs. The downregulation of rno-miR-139 targeted the upregulation of MMP14 and promoted the expression of Sox9. The downregulated rno-miR-139 lead to upregulating Sox9and Collagen II in ectopic cartilage differentiation of BMSCs in vivo. Conclusions Mulberry aqueous extract inhibits rno-miR-139 by upregulating MMP14 to promote chondrogenic differentiation of BMSCs.更多还原