【摘要】 为促进伽师瓜品种保育及种质创新，本研究以伽师瓜常规种和杂交种的种子为材料，采用无菌播种的方式获得无菌苗，探究不同外植体诱导愈伤组织及不定芽分化的条件。结果表明，2种伽师瓜种子最佳消毒方法是：在超净工作台中去壳后，用70%乙醇浸泡30 s, 3%NaClO溶液消毒3 min。诱导效果最好的外植体是5 d无菌苗的近胚轴端子叶。最佳诱导培养基是基本培养基（MS）+1.0 mg·L^-1 6-苄基腺嘌呤（6-BA）,愈伤组织诱导率达100.0%;伽师瓜常规种不定芽分化率达73.3%,伽师瓜杂交种不定芽分化率达66.7%。更多还原

【Abstract】 In order to protect the variety and innovate the germplasm of the Jiashi melon（Cucumis melo L.）, this study obtained aseptic seedlings through aseptic sowing of common and hybrid Jiashi melon seeds, and explored the conditions for inducing callus tissue and adventitious bud differentiation from different explants. The results showed that the optimal disinfection method for two kinds of Jiashi melon seeds was to soak them in 70% ethanol for 30 seconds and 3% NaClO solution for 3 minutes after shelling them on a clean bench. The best inducing explant was cotyledon of the 5-day aseptic seedling near the hypocotyl. The best induction medium was MS+1.0 mg·L^-1 6-BA, with a callus tissue induction rate of 100.0%. The adventitious bud differentiation rate of common Jiashi melon was 73.3%, and that of hybrid Jiashi melon was 66.7%.更多还原