【摘要】 为鉴定引起某豚鼠养殖场发生急性死亡的病原，对疑似细菌感染的豚鼠进行临床观察、病理剖检，并进行细菌分离纯化，对分离菌株进行革兰氏染色、16S rDNA序列分析及药敏试验。结果显示，死亡豚鼠多脏器中均能分离到同一种致病菌，该菌具有明显的β溶血环，革兰氏染色呈阳性，成对或短链状排列；该分离菌株与马链球菌兽疫亚种(MK614224) 16S rDNA同源性高达99.77%。药敏试验结果表明，该分离株对头孢噻呋、多西环素等4种药物敏感；对红霉素、环丙沙星中度敏感；对头孢噻肟、青霉素等15种药物耐药。本试验结果确定了马链球菌兽疫亚种是引起豚鼠急性死亡的病原，该分离株具有较强的耐药性，为后期豚鼠马链球菌兽疫亚种的防治提供了参考依据。更多还原

【Abstract】 In order to identify the causative agent of sudden death of guinea pigs at a farm, clinical observation, pathological dissection and bacterial isolation were conducted on guinea pigs suspected of bacterial infection. Gram staining, 16S rDNA sequencing and antimicrobial susceptibility test were performed following bacterial isolation. The results showed that the same pathogenic bacteria could be isolated from multiple organs of dead guinea pigs, which had obvious β-hemolytic rings, Gram positive staining, and were arranged in pairs or short chains. The 16S rDNA sequence of the isolated bacterial strain had a high homology of 99.77% with that of Streptococcus equi subsp. zooepidemicus(MK614224). The results of antimicrobial susceptibility test showed that the isolated bacterial strain was sensitive to 4 drugs, including ceftiofur and doxycycline; Moderate sensitivite to erythromycin and ciprofloxacin; Resistant to 15 drugs including cefotaxime and penicillin. The results of this experiment confirmed that Streptococcus equi subsp. zooepidemicus was the pathogen causing sudden death of guinea pigs, and the isolated strain showed strong drug resistance, providing a reference for the prevention and control of Streptococcus equi subsp. zooepidemicus.更多还原