【摘要】 目的 探讨特应性皮炎患者皮肤微生物组失调与血清代谢组异常的相互作用，及其对免疫调控的影响。方法 收集48例特应性皮炎患者和20名健康对照者的皮肤拭子和血清样本。采用16S rRNA基因测序分析皮肤微生物组，非靶向代谢组学技术检测血清代谢物。测定血清中炎症因子水平。利用相关性分析和网络构建，探讨微生物与代谢物之间的关系。结果 患者受累皮肤的微生物多样性显著降低，Chao1指数(85.32±15.27)和Shannon指数(2.35±0.48)均低于健康对照组(118.74±16.85和3.28±0.49,P<0.001)。金黄色葡萄球菌丰度显著增加(45.23%±12.45%vs 13.41%±6.42%,P<0.001)。血清中组胺水平升高(倍数变化+1.85,P<0.001),维生素C水平降低(倍数变化-1.45,P=0.004)。金黄色葡萄球菌与组胺呈正相关(r=0.68,P<0.001)。关联网络显示微生物组失调可能通过影响代谢物参与疾病发生。结论 特应性皮炎患者的皮肤微生物组失调与血清代谢组异常密切相关，二者的相互作用可能影响免疫调控，调节微生物群和代谢途径可能成为潜在的治疗策略。更多还原

【Abstract】 Objective To investigate the interaction between skin microbiome dysbiosis and serum metabolome abnormalities in patients with atopic dermatitis and their effects on immune regulation. Methods Skin swabs and serum samples were collected from 48 patients with atopic dermatitis and 20 healthy controls. Skin microbiome was analyzed by 16S rRNA gene sequencing, and serum metabolites were detected by non-targeted metabolomics. Serum levels of inflammatory factors were measured. Correlation analysis and network construction were used to explore the relationship between microorganisms and metabolites. Results The microbial diversity of affected skin was significantly reduced in patients, and the Chao1 index(85.32±15.27) and Shannon index(2.35±0.48) were lower than those in healthy controls(118.74±16.85 and 3.28±0.49,P<0.001). The abundance of Staphylococcus aureus was significantly increased(45.23%±12.45% vs 13.41%±6.42%,P<0.001). Serum histamine levels were increased(fold change +1.85,P<0.001),and vitamin C levels were decreased(fold change-1.45,P=0.004). S. aureus was positively correlated with histamine(r=0.68,P<0.001). The association network showed that microbiome dysbiosis may be involved in the occurrence of the disease by affecting metabolites. Conclusion Skin microbiome dysbiosis in patients with atopic dermatitis is closely related to serum metabolome abnormalities. The interaction between the two may affect immune regulation. Modulating microbiome and metabolic pathways may become a potential therapeutic strategy.更多还原