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基于RIL群体的玉米粒长、粒宽相关QTLs定位与分析

Mapping and Analysis of QTLs Related to Grain Length and Width of Maize Kernels Based on RIL Population

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【作者】 胡静轩; 方家乐; 赵鹏斌; 贾晓艳; 祝丽英; 郭晋杰; 赵永锋;

【Author】 HU Jingxuan;FANG Jiale;ZHAO Pengbin;JIA Xiaoyan;ZHU Liying;GUO Jinjie;ZHAO Yongfeng;College of Agronomy, Hebei Agricultural University/Hebei Branch of National Maize Improvement Center;Natural Resources and Planning Bureau of Xinhe County;

【通讯作者】 赵永锋;

【机构】 河北农业大学农学院/国家玉米改良中心河北分中心; 新河县自然资源和规划局;

【摘要】 以X178和农系531为亲本构建的400份重组自交系(RIL)为实验材料,利用SSR分子标记技术和复合区间作图法(ICIM-ADD)分别对辛集、保定和海南的玉米籽粒性状进行QTL的定位与分析。结果表明,共检测到35个位点,其中关于粒长的位点20个,粒宽的位点15个,分别位于1、2、3、5、7、8、9号染色体上。8号染色体umc1997~umc1960区间内,在3个及以上环境定位到KL8-3位点,表型贡献率最高,为8.34%;粒宽的表型贡献率在4.20%~17.24%之间,其中,在2种不同的环境中分别检测出qKL2-2,qKL3-1,qKL8-1等3个位点。

【Abstract】 400 recombinant inbred lines(RIL) constructed with X178 and Nongji531 as parents were used as experimental materials. The QTL mapping and analysis of maize(Zea mays L.) grain traits in Xinji, Baoding and Hainan were conducted respectively by using SSR molecular marker technology and composite interval mapping method(ICIM-ADD). The results showed that a total of 35 sites were detected, among which 20 sites were related to grain length and 15 sites were related to grain width. It was distributed on chromosomes 1, 2, 3, 5, 7, 8 and 9. The KL8-3 locus was located in 3 or more environments within the range of umc1997 to umc1960 in chromosome 8, and the phenotypic contribution rate was the highest, at 8.34%. The phenotype contribution rate of grain width ranged from 4.20% to 17.24%. Among them, 3 loci, namely qKL2-2,qKL3-1 and qKL8-1, were detected respectively in 2 different environments.

【关键词】 玉米; 籽粒性状; QTL定位;
【Key words】 Zea mays; grain traits; QTL positioning;
【基金】 保定市科技计划项目(2494N022)
  • 【分类号】S513
  • 【下载频次】33
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